Flow Cytometry (FC/FACS)
(FCM staining of human PBMCs using CD5 MAb (CRIS-1).)
Mouse CD5 Monoclonal Antibody | anti-CD5 antibody
CD5 (CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5) (AP)
Gene Names
CD5; T1; LEU1
Reactivity
Human, Monkey
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
CD5; Monoclonal Antibody; CD5 (CD5 antigen (p56 62); LEU1; Ly12; LyA; Lymphocyte antigen T1/Leu-1; Lymphocyte glycoprotein T1/Leu1; T-cell surface glycoprotein CD5) (AP); CD5 antigen (p56 62); T-cell surface glycoprotein CD5; anti-CD5 antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
CRIS-1
Specificity
Recognizes human CD5. Species Crossreactivity: rhesus monkey. Others not-tested.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-CD5 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP)
Application Notes
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
IF: 0.5-1ug/ml
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
Immunogen
Stimulated human leukocytes. Cellular Localization: Cell surface
Conjugate
AP
Positive Control
293T, Ramos, or MOLT-4 Cells. Tonsil.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Flow Cytometry (FC/FACS)
(FCM staining of human PBMCs using CD5 MAb (CRIS-1).)
Flow Cytometry (FC/FACS)
(FCM staining of human PBMCs using CD5 MAb (CRIS-1).)
Product Categories/Family for anti-CD5 antibody
References
1. Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987). 2. Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53(1):41-9. 3. Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992;148(5):1287-93. 4. Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996;5(1):167-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,578 Da
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
UniProt Protein Name
T-cell surface glycoprotein CD5
Protein Family
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1
UniProt Entry Name
CD5_HUMAN
Uniprot Description
CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity
Biological Process: receptor-mediated endocytosis; cell proliferation; T cell costimulation; cell recognition
Research Articles on CD5
Similar Products
Product Notes
The CD5 cd5 (Catalog #AAA6122807) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD5 (CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5) (AP) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD5 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP). IP: 0.5-1ug/500ug protein lysate IF: 0.5-1ug/ml IHC-F: 0.5-1.0ug/ml for 30 minutes at RT Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD5 cd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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