WB (Western Blot)
(Detection of human JAM-A by western blot. Samples: Whole cell lysate from HEK293T (5, 15, and 50 ug) and HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-JAM-A antibody AAA212061 (lot AAA212061-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human JAM-A Polyclonal Antibody | anti-JAM-A antibody
Rabbit anti-JAM-A Antibody, Affinity Purified
Gene Names
F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JAM-A, Antibody; Rabbit anti-JAM-A Antibody, Affinity Purified; junctional adhesion molecule 1; platelet F11 receptor; platelet adhesion molecule 1; PAM-1; junctional adhesion molecule A; JCAM; JAMA; JAM-1; JAM1; JAM; CD321; CD antigen CD321; KAT; JAM-A; Junctional adhesion molecule A; F11 receptor; anti-JAM-A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-JAM-A antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 249 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human JAM-A by western blot. Samples: Whole cell lysate from HEK293T (5, 15, and 50 ug) and HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-JAM-A antibody AAA212061 (lot AAA212061-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human JAM-A by western blot. Samples: Whole cell lysate from HEK293T (5, 15, and 50 ug) and HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-JAM-A antibody AAA212061 (lot AAA212061-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human JAM-A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-JAM-A antibody AAA212061 (lot AAA212061-1) used for IP at 3 ug/mg lysate. JAM-A was not successfully immunoprecipitated by rabbit anti-JAM-A antibody BL10457, which recognizes an upstream epitope. For blotting immunoprecipitated JAM-A, AAA212061 was used at 1.0 ug/ml.Detection: Chemiluminescence with exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human JAM-A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-JAM-A antibody AAA212061 (lot AAA212061-1) used for IP at 3 ug/mg lysate. JAM-A was not successfully immunoprecipitated by rabbit anti-JAM-A antibody BL10457, which recognizes an upstream epitope. For blotting immunoprecipitated JAM-A, AAA212061 was used at 1.0 ug/ml.Detection: Chemiluminescence with exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human JAM-A by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAM-A (Cat. No. AAA212061 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human JAM-A by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAM-A (Cat. No. AAA212061 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-JAM-A antibody
Junctional adhesion molecule A (JAM-A) is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor [taken from NCBI Entrez Gene (Gene ID: 50848)].
Product Categories/Family for anti-JAM-A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,583 Da
NCBI Official Full Name
junctional adhesion molecule A
NCBI Official Synonym Full Names
F11 receptor
NCBI Official Symbol
F11R
NCBI Official Synonym Symbols
JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
NCBI Protein Information
junctional adhesion molecule A; platelet F11 receptor; platelet adhesion molecule 1; junctional adhesion molecule 1
UniProt Protein Name
Junctional adhesion molecule A
UniProt Gene Name
F11R
UniProt Synonym Gene Names
JAM1; JCAM; JAM-A; JAM-1; PAM-1
UniProt Entry Name
JAM1_HUMAN
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Product Notes
The JAM-A f11r (Catalog #AAA212061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JAM-An Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JAM-A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JAM-A f11r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAM-A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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