Mouse anti-Dog CD49d Monoclonal Antibody | anti-CD49d antibody

MOUSE ANTI HUMAN CD49d

Cat. Num. AAA212850

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Dog
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD49d; Monoclonal Antibody; MOUSE ANTI HUMAN CD49d; anti-CD49d antibody

• Host: Mouse
• Reactivity: Dog
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: Bu49
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 195

• Applicable Applications for anti-CD49d antibody: Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.; Immunohistology: For best results MyBioSource recommend HISTAR detection kits.; Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Histology Positive Control Tissue: Human tonsil.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD49d antibody

Mouse anti Human CD49d monoclonal antibody, clone Bu49 recognizes human CD49d, also known as integrin alpha 4 or VLA-4 alpha subunit. CD49d is a 150kDa single pass type I transmembrane glycoprotein. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997). CD49d is expressed on a broad range of cells including lymphocytes, monocytes, eosinophils and thymocytes. CD49d expression may serve as a prognostic parker of survival and disease progression in patients with chronic lymphocytic leukemia and may have therapeutic potential (Bulian et al. 2008) Mouse anti human CD49, clone Bu49 cross-reacts with the canine B-cell leukemia cell line GL-1 and induces homotypic cell aggregation (Ikewaki et al. 2010)

NCBI and Uniprot Product Information

• NCBI GI #: 67191027
• NCBI GeneID: 3676
• NCBI Accession #: NP_000876.3
• NCBI GenBank Nucleotide #: NM_000885.4
• UniProt Accession #: P13612; Q7Z4L6; D3DPG4; AAB25486
• Molecular Weight: 21,536 Da
• NCBI Official Full Name: integrin alpha-4
• NCBI Official Synonym Full Names: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4; 269C wild type; CD49 antigen-like family member D; VLA-4 subunit alpha; antigen CD49D, alpha-4 subunit of VLA-4 receptor; integrin alpha 4; integrin alpha-4 subunit; integrin alpha-IV; very late activation protein 4 receptor, alpha 4 subunit
• UniProt Protein Name: Integrin alpha-4
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D
• UniProt Entry Name: ITA4_HUMAN

NCBI Description

The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA4: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Belongs to the integrin alpha chain family.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31.3

Cellular Component: focal adhesion; cell surface; membrane; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; metal ion binding; fibronectin binding; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; regulation of immune response; cell-matrix adhesion; heart development; receptor clustering; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; B cell differentiation; blood vessel remodeling; leukocyte tethering or rolling; blood coagulation; leukocyte migration