Mouse anti-Human, Dog CD49d Monoclonal Antibody | anti-CD49d antibody

anti-human CD49d-FITC

Cat. Num. AAA666389

• Reactivity: Human, Dog
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD49d; Monoclonal Antibody; anti-human CD49d-FITC; Monoclonal anti-human CD49d (VLA-4)/Biotin; anti-CD49d antibody

• Host: Mouse
• Reactivity: Human, Dog
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BU49
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD49d antibody: Flow Cytometry (FC/FACS), FX
• Performance: Five x 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD49d/Biotin at a concentration of 5 ug/ml. Cells were then washed twice and incubated 45 minutes with 2 degree reagent Streptavidin R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.20 log10 fluorescent when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD49d antibody.
• Conjugation: FITC
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human peripheral myeloma cells
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD49d antibody

Information: Human CD49d molecules are integrin alpha4 chains that are expressed as heterodimers with CD29 (beta1 integrin) or (beta7 integrin) and function as adhesion receptors. CD49d/CD29 is mainly expressed on thymocytes and B cells with increased expression on activated T cells. The ligands for CD49d/CD29 include VCAM-1 and fibronectin. Antibody BU49 recognizes the CD49d molecule of about 145 kd.

References

Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1617-1618. T. Sato, (1995) J Immunol 155: 2938-2947.