WB (Western Blot)
(Detection of human Rad50 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad50 antibody AAA210806 (lot AAA210806-1) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human Rad50 Polyclonal Antibody | anti-Rad50 antibody
Rabbit anti-Rad50 Antibody, Affinity Purified
Gene Names
RAD50; NBSLD; RAD502; hRad50
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Rad50, Antibody; Rabbit anti-Rad50 Antibody, Affinity Purified; RAD502; DNA repair protein RAD50; hRad50; NBSLD; RAD50 homolog, double strand break repair protein; RAD50 double strand break repair protein; anti-Rad50 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Rad50 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Predicted Reactivity
Bovine, Dog, Rabbit, Orangutan, Monkey, Gorilla, Chimpanzee
Immunogen
Between 700 and 750
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Rad50 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad50 antibody AAA210806 (lot AAA210806-1) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human Rad50 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad50 antibody AAA210806 (lot AAA210806-1) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human Rad50 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Rad50 (Cat. No. AAA210806 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Rad50 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Rad50 (Cat. No. AAA210806 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Rad50 antibody
Rad50 is a component of the Mre11-Rad50-Nbs1 (MRN) complex that repairs DNA double strand breaks during DNA damage and homologous recombination and activates the S-phase checkpoint. Rad50 shows structural similarity to the structural maintenance of chromosomes (SMC) family of proteins. Rad50 has been shown to directly interact with BRCA2, TERF2IP, and ATM.
Product Categories/Family for anti-Rad50 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
150kDa
NCBI Official Full Name
DNA repair protein RAD50
NCBI Official Synonym Full Names
RAD50 homolog (S. cerevisiae)
NCBI Official Symbol
RAD50
NCBI Official Synonym Symbols
NBSLD; RAD502; hRad50
NCBI Protein Information
DNA repair protein RAD50
UniProt Protein Name
DNA repair protein RAD50
UniProt Gene Name
RAD50
UniProt Synonym Gene Names
hRAD50
UniProt Entry Name
RAD50_HUMAN
Similar Products
Product Notes
The Rad50 rad50 (Catalog #AAA210806) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Rad50 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rad50 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Rad50 rad50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad50, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.