Mouse anti-Human CD49b Monoclonal Antibody
Mouse Anti-Human CD49b-UNLB
Reactivity
Human
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
CD49b; Monoclonal Antibody; Mouse Anti-Human CD49b-UNLB; Mouse Anti-Human CD49b - Purified; Unlabeled Antibody; Mouse Anti-Human CD49b; anti-CD49b antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Clone Number
HAS-4
Specificity
Human CD49b, Mr 200-210 kDa
CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha 2 chain. CD49b associates noncovalently with CD29, the integrin beta 1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++- dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.
CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha 2 chain. CD49b associates noncovalently with CD29, the integrin beta 1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++- dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.
Form/Format
Purified (UNLB) Antibody
Applicable Applications for anti-CD49b antibody
Flow Cytometry; Immunoprecipitation
Application Notes
Research Application(s): Identification and enumeration of CD49b+ cells by flow cytometry
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
References
1). Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD49b Section, Academic Press, New York, p. 257.
2). Favaloro, E. J., et al. 1990. Br. J. Haem. 74:385.
Mazurov, A. V., et al. 1991. Thromb. Haemost. 66:494.
3). Hemler, M.E. 1990. Annu. Rev. Immunol. 114:365.
4). Takada, Y., and M.E. Hemler. 1989. J. Cell. Biol. 109:397.
2). Favaloro, E. J., et al. 1990. Br. J. Haem. 74:385.
Mazurov, A. V., et al. 1991. Thromb. Haemost. 66:494.
3). Hemler, M.E. 1990. Annu. Rev. Immunol. 114:365.
4). Takada, Y., and M.E. Hemler. 1989. J. Cell. Biol. 109:397.
Similar Products
Product Notes
The CD49b (Catalog #AAA674141) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human CD49b-UNLB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD49b can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. Research Application(s): Identification and enumeration of CD49b+ cells by flow cytometry Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the CD49b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD49b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
