Rat anti-Mouse CD49b Monoclonal Antibody | anti-CD49b antibody
Anti-Mouse CD49b Monoclonal Antibody (Alexa Fluor 488 Conjugated)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD49b; Monoclonal Antibody; Anti-Mouse CD49b Monoclonal Antibody (Alexa Fluor 488 Conjugated); Mouse CD49b; Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; anti-CD49b antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgM
Clone Number
DX5
Form/Format
Liquid; PBS with 0.05% Proclin300, 1% BSA
Sequence Length
1,178
Applicable Applications for anti-CD49b antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 muL of antibody per test (million cells in 100 ul staining volume or per 100 ul of whole blood). Please check your vial before the experiment.
Conjugation
Alexa Fluor 488
Cellular Localization
Membrane
Preparation and Storage
Store at 2-8 degree C. Protected from prolonged exposure to light.
Related Product Information for anti-CD49b antibody
DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin alpha chain also known as alpha2 integrin, VLA-2 alpha chain, and integrin alpha2 chain. CD49b non-covalently associates with CD29 (beta1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.
References
1. Arase H, et al. 2001. J. Immunol. 167:1141. 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 3. Sasaki K, et al. 2003. Int. Immunol. 15:701. 4. Inoue O, et al. 2003. J. Cell Biol. 160:769.
NCBI and Uniprot Product Information
Uniprot Description
Integrin alpha-2/beta-1 is a collagen receptor, being responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides and E-cadherin. Mice homozygous for a null mutation in the alpha-2 die very early in embryogenesis.
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Product Notes
The CD49b itga2 (Catalog #AAA2553988) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD49b Monoclonal Antibody (Alexa Fluor 488 Conjugated) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD49b can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 muL of antibody per test (million cells in 100 ul staining volume or per 100 ul of whole blood). Please check your vial before the experiment. Researchers should empirically determine the suitability of the CD49b itga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD49b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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