WB (Western Blot)
(Detection of human ADA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADA antibody AAA213140 (lot AAA213140-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human ADA/Adenosine Deaminase Polyclonal Antibody | anti-ADA antibody
Rabbit anti-ADA/Adenosine Deaminase Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ADA/Adenosine Deaminase, Antibody; Rabbit anti-ADA/Adenosine Deaminase Antibody, Affinity Purified; adenosine aminohydrolase; adenosine deaminase; Adenosine deaminase; anti-ADA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ADA antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 100 and 150
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ADA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADA antibody AAA213140 (lot AAA213140-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human ADA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADA antibody AAA213140 (lot AAA213140-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ADA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADA antibody AAA213140 (lot AAA213140-1) used for IP at 6 ug per reaction. ADA was also immunoprecipitated by a rabbit anti-ADA antibodies and BL20481. For blotting immunoprecipitated ADA, AAA213140 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human ADA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADA antibody AAA213140 (lot AAA213140-1) used for IP at 6 ug per reaction. ADA was also immunoprecipitated by a rabbit anti-ADA antibodies and BL20481. For blotting immunoprecipitated ADA, AAA213140 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-ADA antibody
The gene ADA encodes the enzyme adenosine deaminase which catalyzes the hydrolysis of adenosine to inosine. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins. Elevated levels of this enzyme have been associated with congenital hemolytic anemia [taken from NCBI Entrez Gene (Gene ID: 100)].
Product Categories/Family for anti-ADA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,764 Da
NCBI Official Full Name
adenosine deaminase
NCBI Official Synonym Full Names
adenosine deaminase
NCBI Official Symbol
ADA
NCBI Protein Information
adenosine deaminase; adenosine aminohydrolase
UniProt Protein Name
Adenosine deaminase
UniProt Gene Name
ADA
UniProt Synonym Gene Names
ADA1
UniProt Entry Name
ADA_HUMAN
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Product Notes
The ADA ada (Catalog #AAA213140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ADA/Adenosine Deaminase Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADA/Adenosine Deaminase can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ADA ada for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADA/Adenosine Deaminase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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