WB (Western Blot)
(Detection of human Septin 10/SEPT10 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Septin 10/SEPT10 antibody AAA213007 (lot AAA213007-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human Septin 10/SEPT10 Polyclonal Antibody | anti-SEPT10 antibody
Rabbit anti-Septin 10/SEPT10 Antibody, Affinity Purified
Gene Names
SEPT10; FLJ11619
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Septin 10/SEPT10, Antibody; Rabbit anti-Septin 10/SEPT10 Antibody, Affinity Purified; septin-10; Septin-10; septin 10; anti-SEPT10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SEPT10 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 404 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Septin 10/SEPT10 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Septin 10/SEPT10 antibody AAA213007 (lot AAA213007-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human Septin 10/SEPT10 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Septin 10/SEPT10 antibody AAA213007 (lot AAA213007-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Septin 10/SEPT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Septin 10/SEPT10 antibody AAA213007 (lot AAA213007-1) used for IP at 6 ug per reaction. Septin 10/SEPT10 was also immunoprecipitated by rabbit anti-Septin 10/SEPT10 antibody For blotting immunoprecipitated Septin 10/SEPT10, AAA213007 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Septin 10/SEPT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Septin 10/SEPT10 antibody AAA213007 (lot AAA213007-1) used for IP at 6 ug per reaction. Septin 10/SEPT10 was also immunoprecipitated by rabbit anti-Septin 10/SEPT10 antibody For blotting immunoprecipitated Septin 10/SEPT10, AAA213007 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-SEPT10 antibody
Septin 10 is a member of the septin family of cytoskeletal proteins with GTPase activity. This protein localizes to the cytoplasm and nucleus and displays GTP-binding and GTPase activit [taken from NCBI Entrez Gene (Gene ID: 151011)].
Product Categories/Family for anti-SEPT10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,120 Da
NCBI Official Full Name
septin-10 isoform 2
NCBI Official Synonym Full Names
septin 10
NCBI Official Symbol
SEPT10
NCBI Official Synonym Symbols
FLJ11619
NCBI Protein Information
septin-10; OTTHUMP00000207015; OTTHUMP00000207016; OTTHUMP00000207017; OTTHUMP00000207018; OTTHUMP00000207019
UniProt Protein Name
Septin-10
UniProt Gene Name
SEPT10
UniProt Entry Name
SEP10_HUMAN
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Product Notes
The SEPT10 sept10 (Catalog #AAA213007) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Septin 10/SEPT10 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Septin 10/SEPT10 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SEPT10 sept10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Septin 10/SEPT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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