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Immunofluorescence (IF)
(Immunofluorescence analysis of human-liver-cancer tissue. 1,CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Mouse anti-Human CD45 Monoclonal Antibody | anti-PTPRC antibody
CD45 (8C1) Mouse mAb
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
CD45; Monoclonal Antibody; CD45 (8C1) Mouse mAb; GP180; L CA; CD45 antigen; anti-PTPRC antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C1-9B8-2H1
Specificity
The antibody detects endogenous CD45 proteins.
Purity/Purification
Affinity purified
Form/Format
PBS, pH7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-PTPRC antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)-Paraffin
Application Notes
IF: 1:50-200
WB: 1:2000
IHC: 1:50-300
WB: 1:2000
IHC: 1:50-300
Source
Human
Immunogen
Synthetic Peptide of CD45
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Membrane
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.
Immunofluorescence (IF)
(Immunofluorescence analysis of human-liver-cancer tissue. 1,CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Immunofluorescence (IF)
(Immunofluorescence analysis of human-liver-cancer tissue. 1,CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CD45 Monoclonal Antibody(12A9) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CD45 Monoclonal Antibody(12A9) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
Western Blot (WB)
(Western blot analysis of Hela, diluted at 1:2000.)
Western Blot (WB)
(Western blot analysis of Hela, diluted at 1:2000.)
Western Blot (WB)
(Western blot detection of CD45 in Jurkat cell lysates using CD45 (8C1) Mouse mAb(1:1000 diluted). Predicted band size:147KDa. Observed band size:180 to 240KDa.)
Western Blot (WB)
(Western blot detection of CD45 in Jurkat cell lysates using CD45 (8C1) Mouse mAb(1:1000 diluted). Predicted band size:147KDa. Observed band size:180 to 240KDa.)
Related Product Information for anti-PTPRC antibody
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW (kDa): 147
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
Protein Family
UniProt Gene Name
Ptprc
UniProt Synonym Gene Names
Ly-5
UniProt Entry Name
PTPRC_MOUSE
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Product Notes
The PTPRC ptprc (Catalog #AAA4751638) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD45 (8C1) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)-Paraffin. IF: 1:50-200 WB: 1:2000 IHC: 1:50-300. Researchers should empirically determine the suitability of the PTPRC ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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