Mouse anti-Human CD44 (Pgp-1) Monoclonal Antibody | anti-Cd44 antibody

Mouse Anti-Human CD44-UNLB

Cat. Num. AAA674135

• Gene Names: Cd44; CD44A; METAA; RHAMM; MGC124941
• Reactivity: Human
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD44 (Pgp-1); Monoclonal Antibody; Mouse Anti-Human CD44-UNLB; Mouse Anti-Human CD44 - Purified; Unlabeled Antibody; Mouse Anti-Human CD44 (Pgp-1); anti-Cd44 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: F10-44-2
• Specificity: Human CD44; CD44, also known as phagocytic glycoprotein-1 (Pgp-1) and the receptor for hyaluronate, exists as a large number of different isoforms resulting from alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells and erythrocytes is a glycosylated type I transmembrane protein. Other variable isoforms containing glycosaminoglycans have molecular weights ranging from 110 to 250 kDa and are widely expressed on hematopoietic and non-hematopoietic cells. CD44 contributes to the adhesion of leukocytes to endothelial cells, stromal cells and the extracellular matrix. It also plays a functional role in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation.1-6
• Form/Format: Purified (UNLB) Antibody
• Sequence Length: 780

• Applicable Applications for anti-Cd44 antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Characterization: To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

References

1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.
4. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD44 Section, Academic Press, New York, p. 240.
5. Goldstein, L.A., and E.C. Butcher. 1990. Immunogenetics 32:389.
6. Lesley, J., R. Hyman, and P.W. Kincade 1993. Adv. Immunol. 54:271.

NCBI and Uniprot Product Information

• NCBI GI #: 19923703
• NCBI GeneID: 25406
• NCBI Accession #: NP_037056.2
• NCBI GenBank Nucleotide #: NM_012924.2
• UniProt Accession #: O08779; O35249; O70509; P26051; Q9EP99; Q9ERN2; Q9ERN3
• Molecular Weight: 85,918 Da
• NCBI Official Full Name: CD44 antigen
• NCBI Official Synonym Full Names: Cd44 molecule
• NCBI Official Symbol: Cd44
• NCBI Official Synonym Symbols: CD44A; METAA; RHAMM; MGC124941
• NCBI Protein Information: CD44 antigen; PGP-1; PGP-I; HUTCH-I; ECMR-III; hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; phagocytic glycoprotein I; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Cell surface glycoprotein CD44 (hyaluronate binding protein)
• UniProt Protein Name: CD44 protein
• Protein Family: CD44 antigen
• UniProt Gene Name: Cd44
• UniProt Synonym Gene Names: CD44
• UniProt Entry Name: O08779_RAT

NCBI Description

adhesion molecule involved in migration, cell fusion and resportion in osteoclasts that also plays a role in cellular metastasis [RGD]

Uniprot Description

CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Receptor, misc.

Cellular Component: Golgi apparatus; focal adhesion; protein complex; cell surface; basolateral plasma membrane; cytoplasm; apical plasma membrane; plasma membrane; integral to membrane; nucleus; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; transmembrane receptor activity; cytokine binding; hyaluronic acid binding; phosphoprotein binding; protein kinase binding; epidermal growth factor receptor binding; hyalurononglucosaminidase activity

Biological Process: cell migration; Wnt receptor signaling pathway; positive regulation of heterotypic cell-cell adhesion; positive regulation of neutrophil apoptosis; negative regulation of caspase activity; blood vessel maturation; response to organic cyclic substance; positive regulation of peptidyl-serine phosphorylation; response to vitamin A; hyaluronan catabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mature B cell apoptosis; ureteric bud branching; cartilage development; regulation of cell growth; inflammatory response; cell adhesion; healing during inflammatory response; neurite development; negative regulation of apoptosis