Testing Data
(Staining of chicken peripheral blood cells with Mouse anti Chicken CD41/61 followed by Goat anti Mouse IgG (H/L):FITC )
Mouse anti-Chicken CD41/CD61 Monoclonal Antibody | anti-CD41/CD61 antibody
Mouse Anti Chicken CD41/CD61
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Staining of chicken peripheral blood cells with Mouse anti Chicken CD41/61 followed by Goat anti Mouse IgG (H/L):FITC )
Testing Data
(Staining of chicken peripheral blood cells with Mouse anti Chicken CD41/61 followed by Goat anti Mouse IgG (H/L):FITC )
Testing Data
(Staining of chicken peripheral blood cells with FITC conjugated Mouse anti Chicken CD41/61 )
Testing Data
(Staining of chicken peripheral blood cells with FITC conjugated Mouse anti Chicken CD41/61 )
Testing Data
(Staining of chicken peripheral blood cells with phycoerythrin conjugated Mouse anti Chicken CD41/CD61 )
Testing Data
(Staining of chicken peripheral blood cells with phycoerythrin conjugated Mouse anti Chicken CD41/CD61 )
Testing Data
(Published Customer image:Mouse anti Chicken CD41/61 antibody, clone 11C3 used for the evaulation of CD41/61 expressing cells in chicken embryo tissue sections by immunofluorescence.CSF1R-transgene expression is restricted to macrophages in MacReporter embryos. (A) CSF1R-mApple+ cells (red) are restricted to the lumen of primitive blood vessels in ubiquitous CAG-eGFP-expressing HH13 stage embryos (green). (B,C) Confocal analysis of transgene expression in HH21 stage CSF1R-mApple embryos indicates that transgene expression is restricted to CD45+ (B, green), CSF1R+ (C, green) cells in the mesenchyme (red arrowheads) and not CD45+ cells budding from the epithelial layer of the dorsal aorta (white arrowheads). Dotted lines mark the blood vessel (BV) lumen. Scale bars in A-C: 100?um. (D-F) Confocal analysis of CSF1R staining (green) of CSF1R-mApple transgene-expressing cells (red) in the mesenchyme tissue of a HH29 embryo. The transgene is expressed in cells (red) that are CD45+ (D, green) and CSF1R+ (E, green), but are CD41/61- (F, green). Scale bars in D-F: 100?um. BV, blood vessel lumen. (G) Scattered eGFP+ cells are found in the embryonic (Emb.) and extra-exbryonic (Ex-Emb) tissues of HH15 MacGreen embryos. Scale bar: 200?mum. (H-J) Colocalization of eGFP+ cells with LysoTracker Red-stained lysosomes in HH33 embryo footplate and in the interdigit region. Inset in J shows the boxed area in more detail. Scale bars in G-J: 200?um.From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141(16):3255-65.)
Testing Data
(Published Customer image:Mouse anti Chicken CD41/61 antibody, clone 11C3 used for the evaulation of CD41/61 expressing cells in chicken embryo tissue sections by immunofluorescence.CSF1R-transgene expression is restricted to macrophages in MacReporter embryos. (A) CSF1R-mApple+ cells (red) are restricted to the lumen of primitive blood vessels in ubiquitous CAG-eGFP-expressing HH13 stage embryos (green). (B,C) Confocal analysis of transgene expression in HH21 stage CSF1R-mApple embryos indicates that transgene expression is restricted to CD45+ (B, green), CSF1R+ (C, green) cells in the mesenchyme (red arrowheads) and not CD45+ cells budding from the epithelial layer of the dorsal aorta (white arrowheads). Dotted lines mark the blood vessel (BV) lumen. Scale bars in A-C: 100?um. (D-F) Confocal analysis of CSF1R staining (green) of CSF1R-mApple transgene-expressing cells (red) in the mesenchyme tissue of a HH29 embryo. The transgene is expressed in cells (red) that are CD45+ (D, green) and CSF1R+ (E, green), but are CD41/61- (F, green). Scale bars in D-F: 100?um. BV, blood vessel lumen. (G) Scattered eGFP+ cells are found in the embryonic (Emb.) and extra-exbryonic (Ex-Emb) tissues of HH15 MacGreen embryos. Scale bar: 200?mum. (H-J) Colocalization of eGFP+ cells with LysoTracker Red-stained lysosomes in HH33 embryo footplate and in the interdigit region. Inset in J shows the boxed area in more detail. Scale bars in G-J: 200?um.From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141(16):3255-65.)
Mouse anti Chicken CD41/61 clone 11C3 is reported to induce thrombocyte activation (Lacoste-Eleaume et al. 1994).
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Product Notes
The CD41/CD61 (Catalog #AAA225650) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Chicken CD41/CD61 reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's CD41/CD61 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CD41/CD61 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD41/CD61, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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