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WB (Western Blot)
(Detection of human CD22 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, Daudi, Jurkat, Raji, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
CD22 recombinant antibody
Rabbit anti-CD22 Recombinant Monoclonal Antibody [BLR214K]
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CD22, Recombinant Antibody; Rabbit anti-CD22 Recombinant Monoclonal Antibody [BLR214K]; sialic acid-binding Ig-like lectin 2; T-cell surface antigen Leu-14; SIGLEC-2; SIGLEC2; CD antigen CD22; B-lymphocyte cell adhesion molecule; BL-CAM; B-cell receptor CD22; CD22 antigen; CD22 molecule; CD22 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR214K
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for CD22 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 20-687 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD22 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, Daudi, Jurkat, Raji, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human CD22 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, Daudi, Jurkat, Raji, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human CD22 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Daudi cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644 lot 1) used for IP at 12 ul/mg lysate. CD22 was also immunoprecipitated by a second antibody against a different epitope of CD22 (BL-2551B-1F2). For blotting immunoprecipitated CD22, AAA213644 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CD22 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Daudi cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644 lot 1) used for IP at 12 ul/mg lysate. CD22 was also immunoprecipitated by a second antibody against a different epitope of CD22 (BL-2551B-1F2). For blotting immunoprecipitated CD22, AAA213644 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry)
(Detection of human CD22 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemisry)
(Detection of human CD22 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human CD22 by immunocytochemistry. Sample: FFPE section of daudi cells. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human CD22 by immunocytochemistry. Sample: FFPE section of daudi cells. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human CD22 (shaded) in Raji cells by flow cytometry. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human CD22 (shaded) in Raji cells by flow cytometry. Antibody: Rabbit anti-CD22 recombinant monoclonal antibody (AAA213644) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for CD22 recombinant antibody
CD22 mediates B-cell B-cell interactions and is involved in the localization of B-cells in lymphoid tissues. CD22 binds sialylated glycoproteins including CD45. It is involved in B cell activation; negative regulation of the B cell receptor signaling pathway; and regulation of endocytosis. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20273].
Product Categories/Family for CD22 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
B-cell receptor CD22 isoform 1
NCBI Official Synonym Full Names
CD22 molecule
NCBI Official Symbol
CD22
NCBI Official Synonym Symbols
SIGLEC2; SIGLEC-2
NCBI Protein Information
B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2
UniProt Protein Name
B-cell receptor CD22
UniProt Gene Name
CD22
UniProt Synonym Gene Names
SIGLEC2; BL-CAM; Siglec-2
UniProt Entry Name
CD22_HUMAN
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Product Notes
The CD22 cd22 (Catalog #AAA213644) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD22 Recombinant Monoclonal Antibody [BLR214K] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD22 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD22 cd22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD22, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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