Mouse anti-Human CD40 Monoclonal Antibody | anti-CD40 antibody

CD40 (Tumor Necrosis Factor Receptor Superfamily Member 5, B Cell Surface Antigen CD40, Bp50, CD40L Receptor, CDw40, TNFRSF5) (FITC)

Cat. Num. AAA6146366

• Gene Names: CD40; p50; Bp50; CDW40; TNFRSF5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD40; Monoclonal Antibody; CD40 (Tumor Necrosis Factor Receptor Superfamily Member 5; B Cell Surface Antigen CD40; Bp50; CD40L Receptor; CDw40; TNFRSF5) (FITC); anti-CD40 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 1G1
• Specificity: Recognizes human CD40.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 1570

• Applicable Applications for anti-CD40 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-151 from human CD40 (AAH64518) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MVRLPLQCVLWGCLLTAVHPEPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHFHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHRSCSPGFGVKQIDICQPHFPKDRGLNLLM
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (42.35kD).)

Testing Data

(Detection limit for recombinant GST tagged CD40 is ~0.1ng/ml as a capture antibody.)

Related Product Information for anti-CD40 antibody

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis.

Product Categories/Family for anti-CD40 antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 40353029
• NCBI GeneID: 958
• NCBI Official Full Name: Homo sapiens CD40 molecule, TNF receptor superfamily member 5, mRNA
• NCBI Official Synonym Full Names: CD40 molecule
• NCBI Official Symbol: CD40
• NCBI Official Synonym Symbols: p50; Bp50; CDW40; TNFRSF5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5
• Protein Family: CD40 ligand

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]