Mouse CD35 Monoclonal Antibody
MAb to CD35
Applications
ELISA, Flow Cytometry, Immunohistochemistry
Synonyms
CD35; Monoclonal Antibody; MAb to CD35; Monoclonal Antibody to CD35; C3b Receptor 160; 190; 220; and 250kDa; anti-CD35 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1, kappa
Specificity
CD35 antigen is composed of 4 allotypes. C3b Receptor (CR1) is present on B lymphocytes, monocytes, myeloid cells, dendritic cells of lymphocyte follicules and renal podocytes.
Form/Format
Purified, Lyophilized; Lyophilized from PBS containing 1mg/ml BSA
Concentration
0.2 mg/ml (prior to lyophilization) (varies by lot)
Applicable Applications for anti-CD35 antibody
Block, EIA/ELISA, Flow Cytometry (FC), Immunohistochemistry - Formalin/Paraffin (IHC-P)
Application Notes
Mechanism of degradation of C3b. Analysis of lymphoid and myeloid differentiation. J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3b dependent rosette formation with lymphocytes.
Fluorescence Microscopy or Flow Cytometry: 2ug/5 x 105 cells/tests.
Immunohistochemistry: Working dilution: 1:25 to 1:50.
Suitable on frozen sections or cell smears and on Bouin's fixed, paraffin-embedded tissues.
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Fluorescence Microscopy or Flow Cytometry: 2ug/5 x 105 cells/tests.
Immunohistochemistry: Working dilution: 1:25 to 1:50.
Suitable on frozen sections or cell smears and on Bouin's fixed, paraffin-embedded tissues.
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Source
Ascites
Immunogen
Purified C3b receptor from human red blood cells
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Reconstitution
Reconstitute with 1ml distilled water.
Preparation and Storage
Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles. The addition of 0.1% sodium azide is recommended for storage up to one month at 2-8°C.
Product Categories/Family for anti-CD35 antibody
References
• Cook J., et al., (1985), "Mouse monoclonal antibodies to the human C3b receptor", Molec. Immunol., 22 #5, 531-539
• Reid, K.B.M., "Complement system protein which interact with C3b or C4d", Immunology Today, 7, 7
• Reid, K.B.M., "Complement system protein which interact with C3b or C4d", Immunology Today, 7, 7
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Product Notes
The CD35 (Catalog #AAA311979) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD35 can be used in a range of immunoassay formats including, but not limited to, Block, EIA/ELISA, Flow Cytometry (FC), Immunohistochemistry - Formalin/Paraffin (IHC-P). Mechanism of degradation of C3b. Analysis of lymphoid and myeloid differentiation. J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3b dependent rosette formation with lymphocytes. Fluorescence Microscopy or Flow Cytometry: 2ug/5 x 105 cells/tests. Immunohistochemistry: Working dilution: 1:25 to 1:50. Suitable on frozen sections or cell smears and on Bouin's fixed, paraffin-embedded tissues. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Researchers should empirically determine the suitability of the CD35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD35, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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