Western Blot (WB)
(Figure 1: Western blot analysis using CD33 mouse mAb against truncated Trx-CD33 recombinant protein (1), truncated CD33 (aa48-258)-His recombinant protein (2) and truncated CD33 (aa18-259)-hIgGFc transfected CHO-K1 cell lysate (3).)
Mouse anti-Human CD33 Monoclonal Antibody | anti-CD33 antibody
CD33 Antibody
Gene Names
CD33; p67; SIGLEC3; SIGLEC-3
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
CD33; Monoclonal Antibody; CD33 Antibody; CD 33; CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33_HUMAN; FLJ00391; gp67; My9; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; Myeloid differentiation antigen CD33; p67; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; Sialic acid-binding Ig-like lectin 3; SIGLEC 3; Siglec-3; SIGLEC3; anti-CD33 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
CD33 antibody detects endogenous levels of total CD33
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
237
Applicable Applications for anti-CD33 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
Purified recombinant fragment of human CD33 expressed in E. Coli
Subcellular Location
Cell Membrane
Tissue Specificity
Monocytic/myeloid lineage cells.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using CD33 mouse mAb against truncated Trx-CD33 recombinant protein (1), truncated CD33 (aa48-258)-His recombinant protein (2) and truncated CD33 (aa18-259)-hIgGFc transfected CHO-K1 cell lysate (3).)
Western Blot (WB)
(Figure 1: Western blot analysis using CD33 mouse mAb against truncated Trx-CD33 recombinant protein (1), truncated CD33 (aa48-258)-His recombinant protein (2) and truncated CD33 (aa18-259)-hIgGFc transfected CHO-K1 cell lysate (3).)
Related Product Information for anti-CD33 antibody
Description: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Subunit Structure: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.
Post-translational Modifications: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
Similarity: Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Subunit Structure: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.
Post-translational Modifications: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
Similarity: Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 39 kDa
Predicted: 40 kDa
Predicted: 40 kDa
NCBI Official Full Name
myeloid cell surface antigen CD33 isoform 2
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
NCBI Protein Information
myeloid cell surface antigen CD33
UniProt Protein Name
Myeloid cell surface antigen CD33
Protein Family
UniProt Gene Name
CD33
UniProt Synonym Gene Names
SIGLEC3; Siglec-3
Uniprot Description
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Research Articles on CD33
Similar Products
Product Notes
The CD33 cd33 (Catalog #AAA9602379) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD33 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD33 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CD33 cd33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD33, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
