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Mouse CD32 Monoclonal Antibody | anti-FCGR2A antibody

CD32 (Fc Gamma RIIa) Mouse Monoclonal Antibody

Gene Names
FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Reactivity
Human.
Others not tested
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
CD32; Monoclonal Antibody; CD32 (Fc Gamma RIIa) Mouse Monoclonal Antibody; CD32A; Fc fragment of IgG low affinity IIa receptor; Fc gamma RIIa; Fc-gamma RII-a; Fc-gamma-RIIa; FCGR2A; FcgRII; Fcr-2; FcRII-a; IGFR2; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17; anti-FCGR2A antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human.
Others not tested
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
8.7
Specificity
This MAb reacts with a CD32 (FcgRII) epitope distinct from that defined by MAb 8.26 and the epitope overlaps with that of MAb 7.30 (cluster 4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
317
Applicable Applications for anti-FCGR2A antibody
Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Cellular Localization
Cell Surface
Immunogen
K562 and FcgRII+L cells
Hu-Chromosome Location
1q23
Positive Control
K-562 cells. Lymph node, tonsil and placenta.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
References
Ierino et al., J. Immunol, 150: 17941803 (1993)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40kDa
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-a isoform 1
NCBI Official Synonym Full Names
Fc fragment of IgG receptor IIa
NCBI Official Symbol
FCGR2A
NCBI Official Synonym Symbols
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-a
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-a
Protein Family
UniProt Gene Name
FCGR2A
UniProt Synonym Gene Names
CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a
UniProt Entry Name
FCG2A_HUMAN

NCBI Description

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]

Uniprot Description

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane

Molecular Function: protein binding

Disease: Malaria, Susceptibility To; Systemic Lupus Erythematosus

Research Articles on FCGR2A

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Product Notes

The FCGR2A fcgr2a (Catalog #AAA439416) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD32 (Fc Gamma RIIa) Mouse Monoclonal Antibody reacts with Human. Others not tested and may cross-react with other species as described in the data sheet. AAA Biotech's CD32 can be used in a range of immunoassay formats including, but not limited to, Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Functional Studies (Order Ab without BSA & Azide) Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (0.5-1ug/ml). Researchers should empirically determine the suitability of the FCGR2A fcgr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD32, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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