Mouse anti-Human CD312 Monoclonal Antibody | anti-CD312 antibody

CD312 (EMR2, Epidermal Growth Factor-Like Module-Containing Mucin-Like Receptor 2, Epidermal Growth Factor Seven Transmembrane, EGF-TM7) (APC)

Cat. Num. AAA6268450

• Gene Names: EMR2; CD312
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by protein A affinity chromatography from tissue culture supernatant.
• Synonyms: CD312; Monoclonal Antibody; CD312 (EMR2; Epidermal Growth Factor-Like Module-Containing Mucin-Like Receptor 2; Epidermal Growth Factor Seven Transmembrane; EGF-TM7) (APC); anti-CD312 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6A362
• Specificity: Recognizes human CD312. Specifically recognizes the stalk region of CD312.
• Purity/Purification: Purified by protein A affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CD312 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA.
• Conjugate: APC
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Product Categories/Family for anti-CD312 antibody

Antibodies; CD Markers

References

1. Kwakkenbos. M.J. et al. (2002). The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells. J. Leukoc. Biol.71:854-862. 2. Stacey. M. et al. (2003). The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chrondroitin sulphate glycosaminoglycans. Blood.102:2916-2924. 3. Kwakkenbos. M.J. et al. (2004). The EGF-TM7 family: a postgenomic view. Immunogenetics. 55:655-666.

NCBI and Uniprot Product Information

• NCBI GI #: 23397681
• NCBI GeneID: 30817
• NCBI Accession #: NP_038475.2
• NCBI GenBank Nucleotide #: NM_013447.3
• UniProt Accession #: Q9UHX3; Q8NG96; Q9Y4B1; E9PBR1; E9PEL6; E9PFQ5; E9PG91
• Molecular Weight: 90,472 Da
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 2 isoform a
• NCBI Official Synonym Full Names: egf-like module containing, mucin-like, hormone receptor-like 2
• NCBI Official Symbol: EMR2
• NCBI Official Synonym Symbols: CD312
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 2
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 2
• UniProt Gene Name: EMR2
• UniProt Entry Name: EMR2_HUMAN

NCBI Description

This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

EMR2: Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell migration; cell adhesion; inflammatory response