Mouse anti-Human CD31 Monoclonal Antibody | anti-CD31 antibody

Anti-Hu CD31 Alexa Fluor488

• Gene Names: PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD31; Monoclonal Antibody; Anti-Hu CD31 Alexa Fluor488; PECAM-1; EndoCAM; GPIIA; PECA1; platelet and endothelial cell adhesion molecule 1; anti-CD31 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: MEM-05
• Specificity: The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD31 antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor488
• Immunogen: Leukocytes of patient suffering from LGL-type leukaemia
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD31 (MEM-05) Alexa Fluor 488 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human monocytes (red-filled) from CD31 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD31 (MEM-05) Alexa Fluor 488 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD31 antibody

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multiFunctional Application (FUNC)tional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

References

*Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J Immunol. 2001 Feb 15;166(4):2364-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/11160294dopt=,*Car?an? T, S?ftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocalteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies. PLoS One. 2012;7(12):e52815. doi: 10.1371/journal.pone.0052815., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=gruionu+c,*Kalina T, Bakardjieva M, Blom M, Perez-Andres M, Barendregt B, Kanderová V, Bonroy C, Philippé J, Blanco E, Pico-Knijnenburg I, Paping JH, Wolska-Ku?nierz B, Pac M, Tkazcyk J, Haerynck F, Akar HH, Formánková R, Freiberger T, Svato? M, Šedivá A, Arriba-Méndez S, Orfao A, van Dongen JJ, van der Burg M: EuroFlow standardized approach to diagnostic immunopheneotyping of severe PID innNewborns and young children. Front Immunol. 2020 Mar 19;11:371., URL: https://pubmed.ncbi.nlm.nih.gov/32265901/
*Wong MX, Hayball JD, Jackson DE: PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells. Mol Immunol. 2007 Oct 29, URL: https://www.ncbi.nlm.nih.gov/pubmed/17977600ordin,*Woodfin A, Voisin MB, Nourshargh S: PECAM-1: a multi-Functional Application (FUNC)tional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol. 2007 Dec;27(12):2514-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/17872453ordin,*Wee JL, Jackson DE: The Ig-ITIM superfamily member PECAM-1 regulates the "outside-in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005 Dec 1;106(12):3816-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/16081692ordin,*Jackson DE: The unfolding tale of PECAM-1. FEBS Lett. 2003 Apr 10;540(1-3):7-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/12681475ordin,*Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) acts as a regulator of B-cell development, B-cell antigen receptor (BCR)-mediated activation, and autoimmune disease. Blood. 2002 Jul 1;100(1):184-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/12070026ordin,*Newman DK, Hamilton C, Newman PJ: Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires Functional Application (FUNC)tional ITIMs, SHP-2, and p56(lck). Blood. 2001 Apr 15;97(8):2351-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/11290597ordin

NCBI and Uniprot Product Information

• NCBI GI #: 313760624
• NCBI GeneID: 5175
• NCBI Accession #: NP_000433.4
• NCBI GenBank Nucleotide #: NM_000442.4
• UniProt Accession #: P16284; Q6LDA9; Q8TBH1; Q96RF5; Q96RF6; Q9NP65; Q9NPB7; Q9NPG9; Q9NQS9; Q9NQT0; Q9NQT1; Q9NQT2
• Molecular Weight: 82,536 Da
• NCBI Official Full Name: platelet endothelial cell adhesion molecule
• NCBI Official Synonym Full Names: platelet/endothelial cell adhesion molecule 1
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• NCBI Protein Information: platelet endothelial cell adhesion molecule; CD31 antigen
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: PECAM1
• UniProt Synonym Gene Names: PECAM-1
• UniProt Entry Name: PECA1_HUMAN

NCBI Description

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Uniprot Description

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis