Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody
APC-Cyanine7 Anti-Human CD3 (UCHT1)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Affinity Purified
Synonyms
CD3; Monoclonal Antibody; APC-Cyanine7 Anti-Human CD3 (UCHT1); anti-CD3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
UCHT1
Purity/Purification
Affinity Purified
Form/Format
Liquid. 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3,0.1% gelatin, pH7.2
Applicable Applications for anti-CD3 antibody
Flow Cytometry (FC/FACS)
Conjugation
APC-Cyanine7 conjugated
Preparation and Storage
Shipping Condition: Ice Packs
Related Product Information for anti-CD3 antibody
The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation (use format suitable for functional assays). A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein. Also, the UCHT1 antibody is reported to be cross-reactive with chimpanzee and has been used for phenotypic analysis of expression by Flow Cytometry (FC/FACS)ometry; however the antibody is reported to be unsuitable for induction of T cell activation in this species (Bibollet-Ruche et al. 2009. J. Virol. 82: 10271-10278).
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Product Notes
The CD3 (Catalog #AAA4165302) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The APC-Cyanine7 Anti-Human CD3 (UCHT1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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