Flow Cytometry (FC/FACS)
(Fig-1: Cell Surface flow analysis of mouse CD3 in mouse splenocytes using 0.5 ug/10^6 cells. Green represents isotype control; red represents FITC conjugate mouse CD3 antibody.)
Hamster anti-Mouse CD3 Monoclonal Antibody | anti-CD3 antibody
Monoclonal Antibody to mCD3 (Clone: 145-2C11) FITC conjugated
Gene Names
Cd247; Cd3; T3z; Cd3h; Cd3z; Tcrk; Tcrz; Cd3-eta; Cd3zeta; AW552088; Cd3-zeta; 4930549J05Rik; A430104F18Rik
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
PurifiedProtein G Chromatography
Synonyms
CD3; Monoclonal Antibody; Monoclonal Antibody to mCD3 (Clone: 145-2C11) FITC conjugated; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD247; Cd3z; Tcrz; anti-CD3 antibody
Host
Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
145-2C11
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
0.2 mg/ml in Tris buffer containing 0.05% Azide
Sequence Length
164
Applicable Applications for anti-CD3 antibody
Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry: 0.5-1 ug/10^6 cells
Immunogen Information
Mouse BM10-37 cytotoxic T lymphocytes were used as immunogen for this antibody.
Antigen
CD3
Conjugate
FITC
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
Flow Cytometry (FC/FACS)
(Fig-1: Cell Surface flow analysis of mouse CD3 in mouse splenocytes using 0.5 ug/10^6 cells. Green represents isotype control; red represents FITC conjugate mouse CD3 antibody.)
Flow Cytometry (FC/FACS)
(Fig-1: Cell Surface flow analysis of mouse CD3 in mouse splenocytes using 0.5 ug/10^6 cells. Green represents isotype control; red represents FITC conjugate mouse CD3 antibody.)
Product Categories/Family for anti-CD3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,339 Da
NCBI Official Full Name
T-cell surface glycoprotein CD3 zeta chain
NCBI Official Synonym Full Names
CD247 antigen
NCBI Official Symbol
Cd247
NCBI Official Synonym Symbols
Cd3; T3z; Cd3h; Cd3z; Tcrk; Tcrz; Cd3-eta; Cd3zeta; AW552088; Cd3-zeta; 4930549J05Rik; A430104F18Rik
NCBI Protein Information
CD247 antigen
UniProt Protein Name
T-cell surface glycoprotein CD3 zeta chain
Protein Family
UniProt Gene Name
Cd247
UniProt Synonym Gene Names
Cd3z; Tcrz
Uniprot Description
CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1 H2.3|1 73.14 cM
Cellular Component: alpha-beta T cell receptor complex; cytoplasm; plasma membrane; T cell receptor complex
Molecular Function: identical protein binding; protein binding
Biological Process: cell surface receptor linked signal transduction; T cell receptor signaling pathway
Research Articles on CD3
Similar Products
Product Notes
The CD3 cd247 (Catalog #AAA668426) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to mCD3 (Clone: 145-2C11) FITC conjugated reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow Cytometry: 0.5-1 ug/10^6 cells. Researchers should empirically determine the suitability of the CD3 cd247 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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