Rat anti-Mouse CD29 Monoclonal Antibody | anti-CD29 antibody

Anti-Mouse CD29 Monoclonal Antibody (FITC Conjugated) [KMI6]

Cat. Num. AAA2558039

• Gene Names: Itgb1; CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD29; Monoclonal Antibody; Anti-Mouse CD29 Monoclonal Antibody (FITC Conjugated) [KMI6]; Integrin beta-1; ITB1; Itgb1; Fibronectin receptor subunit beta; VLA-4 subunit beta; anti-CD29 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: KMI6
• Form/Format: PBS with 0.05% Proclin300, 1% BSA
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 798

• Applicable Applications for anti-CD29 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 6 ug per 10⁶ cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
• Conjugation: FITC
• Preparation and Storage: Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse CD29 Monoclonal Antibody(FITC Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD29 antibody

The KMI6 antibody reacts with the 130-kDa integrin beta1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin achains. With a1 through a6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with av (CD51), it forms avbeta1 integrin. It also associates with the integrin a7, a8, and a9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, smooth muscle, epithelia, and oocytes. The epitope recognized by the KMI6 mAb can be detected by immunoblot in unreduced extracts from a variety of cells and tissues having a number of different beta1-integrin heterodimer combinations. However, the epitope is not well expressed on the surface of the majority of hematopoietic cells. It is detected on the surface of fetal liver cells, some bone marrow stromal cells, and some cultured hematopoietic cell lines, including P815 and PA6. Surface expression of the CD29 epitope recognized by mAb KMI6 on thymocytes and T lymphocytes appears to be developmentally regulated, and KMI6 antibody enhances the adhesion of KMI6 thymocytes to fibronectin and laminin.

NCBI and Uniprot Product Information

• NCBI GI #: 124964
• NCBI GeneID: 16412
• UniProt Accession #: P09055
• NCBI Official Full Name: Integrin beta-1
• NCBI Official Synonym Full Names: integrin beta 1 (fibronectin receptor beta)
• NCBI Official Symbol: Itgb1
• NCBI Official Synonym Symbols: CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik
• NCBI Protein Information: integrin beta-1
• UniProt Protein Name: Integrin beta-1
• UniProt Gene Name: Itgb1
• UniProt Entry Name: ITB1_MOUSE

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Membrane protein, integral; Cell adhesion; Receptor, misc.; Motility/polarity/chemotaxis; Cell surface

Cellular Component: focal adhesion; cell surface; dendritic spine; integral to membrane; acrosome; intercellular junction; lipid raft; cell projection; adherens junction; hemidesmosome; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; basement membrane; synapse; cytoplasmic vesicle; neuromuscular junction; cell junction; integrin complex; receptor complex; sarcolemma; endosome; filopodium; external side of plasma membrane

Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; receptor activity; actin binding; alpha-actinin binding; peptide binding; protein kinase binding; collagen binding; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; protein complex binding; cell adhesion molecule binding; glycoprotein binding; kinase binding; receptor binding

Biological Process: positive regulation of apoptosis; axon extension; regulation of cell cycle; multicellular organismal development; cell-matrix adhesion; cell fate specification; positive regulation of endocytosis; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; germ cell migration; positive regulation of cell proliferation; tissue homeostasis; visual learning; cell adhesion; negative regulation of cell projection organization and biogenesis; neurite development; integrin-mediated signaling pathway; cardiac muscle development; cell migration; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; formation of radial glial scaffolds; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; negative regulation of cell differentiation; negative regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; negative regulation of Rho protein signal transduction; calcium-independent cell-matrix adhesion; stress fiber formation; positive regulation of neuron differentiation; leukocyte tethering or rolling; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; positive regulation of cell migration