Mouse anti-Chicken CD28 Monoclonal Antibody | anti-Cd28 antibody

Mouse Anti-Chicken CD28-PE

Cat. Num. AAA673895

• Gene Names: Cd28; Cd28
• Reactivity: Chicken
• Applications: Flow Cytometry, Immunoprecipitation
• Synonyms: CD28; Monoclonal Antibody; Mouse Anti-Chicken CD28-PE; Mouse Anti-Chicken CD28 - Phycoerythrin-Labeled; Mouse Anti-Chicken CD28; anti-Cd28 antibody

• Host: Mouse
• Reactivity: Chicken
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: AV7
• Specificity: Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28; Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma delta + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V beta 1- and V beta 2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.2 MAb AV7 also reacts with turkey CD28 as demonstrated by flow cytometry and immunoprecipitation/Western blotting.3
• Form/Format: R - phycoerythrin (R - PE) Conjugate
• Sequence Length: 63

• Applicable Applications for anti-Cd28 antibody: Flow Cytometry; Immunoprecipitation
• Application Notes: Research Application(s): In vitro T-cell costimulation; ; Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this data sheet.
• Preparation and Storage: The purified antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added; store at 2-8 degree C. ; The FITC conjugate is supplied as 0.5 mg in 1.0 mL of phosphate buffered saline, pH 7.4, containing 0.1% sodium azide as preservative (PBS/NaN3); store at 2-8 degree C. ; The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3; store at 2-8 degree C. ; The R-PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent; store at 2-8 degree C. ; All products should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Young, J.R., T.F. Davison, C.A. Tregaskes, M.C. Rennie, and O. Vainio. 1994. J. Immunol. 152:3848.
2. Arstila, T.P., O. Vainio, and O. Lassila. 1994. Scand J. Immunol. 40:368.

NCBI and Uniprot Product Information

• NCBI GI #: 192485
• NCBI GeneID: 12487
• UniProt Accession #: P31041; Q8CDB3
• NCBI Official Full Name: CD28
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Official Synonym Symbols: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; OTTMUSP00000002157
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Entry Name: CD28_MOUSE

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: membrane; integral to membrane; plasma membrane; immunological synapse; lipid raft; external side of plasma membrane

Molecular Function: protein homodimerization activity; protease binding; SH3/SH2 adaptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of mitosis; T cell activation; positive regulation of interleukin-2 biosynthetic process; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of inflammatory response to antigenic stimulus; T cell costimulation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; immune response; positive regulation of alpha-beta T cell proliferation; regulatory T cell differentiation