Hamster anti-Mouse CD28 Monoclonal Antibody | anti-CD28 antibody

Anti-Mouse CD28 Monoclonal Antibody (PE Conjugated) [37.51]

Cat. Num. AAA2558191

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD28; Monoclonal Antibody; Anti-Mouse CD28 Monoclonal Antibody (PE Conjugated) [37.51]; T-cell-specific surface glycoprotein CD28; Cd28; anti-CD28 antibody

• Host: Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 37.51
• Form/Format: PBS with 0.05% Proclin300, 1% BSA
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 218

• Applicable Applications for anti-CD28 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 0.2 ug per 10⁶ cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
• Conjugation: PE
• Preparation and Storage: Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse CD28 Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD28 antibody

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

NCBI and Uniprot Product Information

• NCBI GI #: 161484597
• NCBI GeneID: 12487
• NCBI Accession #: NP_031668.3
• NCBI GenBank Nucleotide #: NP_031668.3
• UniProt Accession #: P31041
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: Cd28

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.