Rat CD273 Monoclonal Antibody | anti-CD273 antibody

RAT ANTI MOUSE CD273

Cat. Num. AAA214323

• Gene Names: Pdcd1lg2; Btdc; B7-DC; PD-L2; F730015O22Rik
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD273; Monoclonal Antibody; RAT ANTI MOUSE CD273; anti-CD273 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: TY25
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 247

• Applicable Applications for anti-CD273 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD273 antibody

Rat anti Mouse CD273 antibody, clone TY25 recognises mouse CD273, a 42kD member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Antibodies produced by clone TY25 are reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al.). Removal of Sodium Azide is recommended prior to use in functional assays. MyBioSource recommend the use of EQU003 for this purpose.

NCBI and Uniprot Product Information

• NCBI GI #: 10946740
• NCBI GeneID: 58205
• NCBI Accession #: NP_067371.1
• NCBI GenBank Nucleotide #: NM_021396.2
• UniProt Accession #: Q9WUL5
• Molecular Weight: 27,820 Da
• NCBI Official Full Name: programmed cell death 1 ligand 2
• NCBI Official Synonym Full Names: programmed cell death 1 ligand 2
• NCBI Official Symbol: Pdcd1lg2
• NCBI Official Synonym Symbols: Btdc; B7-DC; PD-L2; F730015O22Rik
• NCBI Protein Information: programmed cell death 1 ligand 2; PD-1 ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; butyrophilin-like protein; programmed death ligand 2
• UniProt Protein Name: Programmed cell death 1 ligand 2
• UniProt Gene Name: Pdcd1lg2
• UniProt Synonym Gene Names: B7dc; Btdc; Cd273; Pdl2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC
• UniProt Entry Name: PD1L2_MOUSE

Uniprot Description

PDCD1LG2: Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: negative regulation of T cell proliferation; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; positive regulation of T cell proliferation