ELISA (EIA)
(Sandwich ELISA analysis of human CD258 using Mouse anti Human CD258 (MBS226109) as a capture reagent and biotinylated Mouse anti Human CD258 as a detection reagent with purified recombinant CD258 as the antigen. Detection was performed using HR)
Mouse anti-Human CD258 Monoclonal Antibody | anti-CD258 antibody
Mouse anti Human CD258
0.09% Sodium Azide (NaN3)
Purified IgG - liquid
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA (EIA)
(Sandwich ELISA analysis of human CD258 using Mouse anti Human CD258 (MBS226109) as a capture reagent and biotinylated Mouse anti Human CD258 as a detection reagent with purified recombinant CD258 as the antigen. Detection was performed using HR)
ELISA (EIA)
(Sandwich ELISA analysis of human CD258 using Mouse anti Human CD258 (MBS226109) as a capture reagent and biotinylated Mouse anti Human CD258 as a detection reagent with purified recombinant CD258 as the antigen. Detection was performed using HR)
CD258 is primarily expressed by activated T cells, activated natural killer cells and immature dentritic cells. It is initially synthesized as a type II transmembrane protein, which can be proteolytically cleaved to release a soluble bioactive form (Ware & Sedý 2011).
The purified Mouse anti Human CD258 antibody, clone D04-8A4 can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human CD258 antibody, clone B01-9B3 as the detection antibody.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cytokine; Inhibitor
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: extracellular space; cytoplasm; plasma membrane; integral to membrane
Molecular Function: protein binding; caspase inhibitor activity; cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: release of cytoplasmic sequestered NF-kappaB; T cell proliferation; T cell activation; apoptosis; T cell costimulation; T cell homeostasis; immune response; negative regulation of caspase activity; signal transduction; positive regulation of myoblast differentiation
Research Articles on CD258
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Product Notes
The CD258 tnfsf14 (Catalog #AAA226109) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human CD258 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD258 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the CD258 tnfsf14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD258, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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