Mouse CD243 Monoclonal Antibody | anti-CD243 antibody

MOUSE ANTI HUMAN CD243

Cat. Num. AAA214427

• Gene Names: ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
• Synonyms: CD243; Monoclonal Antibody; MOUSE ANTI HUMAN CD243; anti-CD243 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: UIC2
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1280

• Applicable Applications for anti-CD243 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Histology Positive Control Tissue: Human kidney.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD243 antibody

Mouse anti Human CD243, clone UIC2 recognizes an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a multi pass transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, containing two ABC transporter type 1 domains and two ABC transporter domains. CD243 acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell (Park et al. 2003). Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase. Exposure of monocytes, which do not constitutively express CD243 leads to an increase in surface expression and a significant enhancement of its substrate  efflux activity. This increase in cell surface expression and efflux activity has implications for the drug resistance actions of CD243, not allowing concentrations of therapeutic agents such as cyclosporine (ritonavir) to reach beneficial levels in cells (Tempestilli et al. 2014).

NCBI and Uniprot Product Information

• NCBI GI #: 42741659
• NCBI GeneID: 5243
• NCBI Accession #: NP_000918.2
• NCBI GenBank Nucleotide #: NM_000927.4
• UniProt Accession #: P08183; Q12755; Q14812; A8K294; B5AK60
• Molecular Weight: 134,032 Da
• NCBI Official Full Name: multidrug resistance protein 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 1
• NCBI Official Symbol: ABCB1
• NCBI Official Synonym Symbols: CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
• NCBI Protein Information: multidrug resistance protein 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance
• UniProt Protein Name: Multidrug resistance protein 1
• UniProt Gene Name: ABCB1
• UniProt Synonym Gene Names: MDR1; PGY1
• UniProt Entry Name: MDR1_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB1: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Interacts with PSMB8.

Protein type: Cell adhesion; Transporter, ABC family; Hydrolase; EC 3.6.3.44; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q21.12

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; ATPase activity, coupled to transmembrane movement of substances; transporter activity; ATP binding; xenobiotic-transporting ATPase activity

Biological Process: response to drug; transport; xenobiotic transport; multidrug transport; G2/M transition of mitotic cell cycle; transmembrane transport

Disease: Colchicine Resistance; Inflammatory Bowel Disease 13