Rat anti-Mouse CD23 (Fc epsilon RII) Monoclonal Antibody

Rat Anti-Mouse CD23/FCeRII-LE/AF

Cat. Num. AAA673269

• Reactivity: Mouse
• Applications: Flow Cytometry, Immunoprecipitation
• Synonyms: CD23 (Fc epsilon RII); Monoclonal Antibody; Rat Anti-Mouse CD23/FCeRII-LE/AF; Rat Anti-Mouse CD23/FCeRII - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD23 (Fc epsilon RII); anti-CD23 (Fc epsilon RII) antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone Number: 2G8
• Specificity: CD23 low affinity IgE Fc receptor; The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments.^1 It is expressed on most mature, conventional B cells (but not on peritoneal CD5^+ B cells), and can also be found on the surface of T cells, macrophages and platelets.^1-6 It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation.^3 The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors.^2-6 CD23 has been described as a "membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.^6
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)

• Applicable Applications for anti-CD23 (Fc epsilon RII) antibody: Flow Cytometry; Immunoprecipitation
• Application Notes: Research Application(s): Differentiation of B cell subpopulations by cell sorting
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Rao, M., W. Lee, and D. Conrad. 1987. J. Immunol. 138:1845.
2. Cong Y.Z., Rabin E. and Wortis H.H. 1991. International Immunology. 3:467.
3. Conrad DH. 1991. Monographs in Allergy. 29:9.
4. Vercelli D.and Geha RS. 1989. Annals of Allergy. 63:4.
5. Yu, P., Kosco-Vilbios, M., Richards, M., Kohler, G. and Lamers, M.C. 1994. Nature 369:753.
6. Bonnefoy, J.-Y., Lecoanet-Henchoz, S., Aubry, J.P., Gauchat, J.F. and Graber, P. 1995. Current Opinions in Immunol. 7:355.