Mouse anti-Human CD23 Monoclonal Antibody | anti-CD23 antibody

Mouse anti-Human CD23, Purified mAb

Cat. Num. AAA8500637

• Gene Names: Fcer2; CD23; Fcer2a
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD23; Monoclonal Antibody; Mouse anti-Human CD23; Purified mAb; anti-CD23 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4AEBVCS2
• Form/Format: Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
• Sequence Length: 309

• Applicable Applications for anti-CD23 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube; ; 2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature; ; 3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×); ; 4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.; 7.Repeat step 5.; 8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).; [PBS wash buffer: PBS +1% FBS +0.1% NaN3]; [Cell fixation: 2% formaldehyde solution]
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Related Product Information for anti-CD23 antibody

The 4AEBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulatedupon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23.

References

1.Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. 2.McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York. 3.Bernard, A., et al. eds. (1981). Leukocyte Typing. Springer-Verlag.

NCBI and Uniprot Product Information

• NCBI GI #: 313673
• NCBI GeneID: 171075
• UniProt Accession #: Q63097
• Molecular Weight: 35,126 Da
• NCBI Official Full Name: CD23
• NCBI Official Synonym Full Names: Fc fragment of IgE receptor II
• NCBI Official Symbol: Fcer2
• NCBI Official Synonym Symbols: CD23; Fcer2a
• NCBI Protein Information: low affinity immunoglobulin epsilon Fc receptor
• UniProt Protein Name: CD23 protein
• UniProt Gene Name: Fcer2

NCBI Description

cell surface receptor; binds innumoglobulin (Ig)E and IgA antibodies [RGD, Feb 2006]