Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse Ly9(CD229) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse Ly9(CD229), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Mouse CD229 Monoclonal Antibody | anti-LY9 antibody
Recombinant Anti-CD229 Antibody, Rabbit Monoclonal
Gene Names
Ly9; Ly-9; T100; CD229; Lgp100; SLAMF3; AI893573
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
CD229; Monoclonal Antibody; Recombinant Anti-CD229 Antibody; Rabbit Monoclonal; LY9/CD229/SLAMF3 Antibody; Rabbit MAb; lymphocyte antigen 9; Anti-AI893573 Antibody; Anti-CD229 Antibody; Anti-Lgp100 Antibody; Anti-Ly-9 Antibody; Anti-SLAMF3 Antibody; Anti-T100 Antibody; anti-LY9 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
002
Specificity
Mouse LY9/CD229/SLAMF3
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-LY9 antibody
Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:20-1:100
FC/FACS/FCM: 1:25-1:100
FC/FACS/FCM: 1:25-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse LY9/CD229/SLAMF3 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LY9/CD229/SLAMF3 (rM LY9/CD229/SLAMF3).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse Ly9(CD229) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse Ly9(CD229), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse Ly9(CD229) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse Ly9(CD229), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse Ly9 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse Ly9 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse Ly9 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse Ly9 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).)
Product Categories/Family for anti-LY9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,143 Da
NCBI Official Full Name
T-lymphocyte surface antigen Ly-9 isoform 1
NCBI Official Synonym Full Names
lymphocyte antigen 9
NCBI Official Symbol
Ly9
NCBI Official Synonym Symbols
Ly-9; T100; CD229; Lgp100; SLAMF3; AI893573
NCBI Protein Information
T-lymphocyte surface antigen Ly-9
UniProt Protein Name
T-lymphocyte surface antigen Ly-9
Protein Family
UniProt Gene Name
Ly9
UniProt Synonym Gene Names
Ly-9; SLAMF3
UniProt Entry Name
LY9_MOUSE
Uniprot Description
Function: May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction.
Subcellular location: Membrane; Single-pass type I membrane protein.
Tissue specificity: Lymphocytes.
Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Research Articles on LY9
Similar Products
Product Notes
The LY9 ly9 (Catalog #AAA8111676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD229 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD229 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the LY9 ly9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD229, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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