Flow Cytometry
(Flow Cytometric analysis using CD205 antibodyStaining of human peripheral blood lymphocytes (left) and mature dendritic cells (right) with 0.015 ug of Mouse IgG2b kappa Isotype Control (biotin) (open histogram) or 0.015 ug of CD205 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Mouse CD205 Monoclonal Antibody | anti-CD205 antibody
CD205 antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD205; Monoclonal Antibody; CD205 antibody (biotin); Monoclonal CD205; Anti-CD205; DEC-205; CD-205; CD 205; DEC205; anti-CD205 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
MG38
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-CD205 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.25 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
Biological Significance
The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor. CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo. CD205 is highly expressed by dendritic cells within the T cell areas of lymphoid tissues, particularly on CD8 DCs that are believed to play a role in cross-priming. Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs and at intermediate intensity on immature human DCs. All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry
(Flow Cytometric analysis using CD205 antibodyStaining of human peripheral blood lymphocytes (left) and mature dendritic cells (right) with 0.015 ug of Mouse IgG2b kappa Isotype Control (biotin) (open histogram) or 0.015 ug of CD205 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry
(Flow Cytometric analysis using CD205 antibodyStaining of human peripheral blood lymphocytes (left) and mature dendritic cells (right) with 0.015 ug of Mouse IgG2b kappa Isotype Control (biotin) (open histogram) or 0.015 ug of CD205 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry
(Flow Cytometric analysis using CD205 antibodyStaining of human peripheral blood lymphocytes (left) and mature dendritic cells (right) with 0.015 ug of Mouse IgG2b kappa Isotype Control (biotin) (open histogram) or 0.015 ug of CD205 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry
(Flow Cytometric analysis using CD205 antibodyStaining of human peripheral blood lymphocytes (left) and mature dendritic cells (right) with 0.015 ug of Mouse IgG2b kappa Isotype Control (biotin) (open histogram) or 0.015 ug of CD205 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Related Product Information for anti-CD205 antibody
Mouse monoclonal CD205 antibody (biotin)
Product Categories/Family for anti-CD205 antibody
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Product Notes
The CD205 (Catalog #AAA832258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD205 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.25 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD205 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD205, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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