Mouse anti-Human CD166 Monoclonal Antibody | anti-CD166 antibody

anti-human CD166-FITC

Cat. Num. AAA666762

• Gene Names: ALCAM; MEMD; CD166
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD166; Monoclonal Antibody; anti-human CD166-FITC; Monoclonal anti-human CD166 (ALCAM)/FITC; anti-CD166 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A6
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: Volume: 0.2ml (varies by lot)
• Sequence Length: 133

• Applicable Applications for anti-CD166 antibody: Flow Cytometry (FC/FACS)
• Application Notes: 1:50 (or 1.6ul of concentrated stock per 5 x 105 cell test)
• Performance: Five x 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD166/FITC at a 1:50 dilution factor (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.6 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD166 antibody.
• Conjugation: FITC
• Production: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/IgG molar ratio of 7.4.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human thymic epithelial cells
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD166 antibody

Information: Human CD166 is a type 1 glycoprotein expressed on activated T cells, B cells and monocytes and appears to be the ligand for CD6 (1,2). Human CD166 may be important for activation of T cells. Antibody 3A6 recognizes the CD166 molecule of 100 kd. Antibody 3A6 blocks binding of CD6 to CD166.

References

(1). M.A. Bowen, et al, (1995) J Exp Med 181: 2213-2220. (2). M.A. Bowen, et al, (1996) J Biol Chem 271: 17390-17396. (3). Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 459-465.

NCBI and Uniprot Product Information

• NCBI GI #: 343168776
• NCBI GeneID: 214
• NCBI Accession #: NP_001230212.1
• NCBI GenBank Nucleotide #: NM_001243283.1
• Molecular Weight: 33,878 Da
• NCBI Official Full Name: CD166 antigen isoform 4
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: ALCAM
• NCBI Official Synonym Symbols: MEMD; CD166
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: ALCAM
• UniProt Synonym Gene Names: MEMD
• UniProt Entry Name: CD166_HUMAN

NCBI Description

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion