Testing Data
(Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse anti human CD163 . All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
Mouse anti-Human CD163 Monoclonal Antibody | anti-CD163 antibody
Mouse Anti Human CD163: RPE
Testing Data
(Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse anti human CD163 . All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse anti human CD163 . All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. RPE conjugated mouse anti CD14 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti CD14 and FITC conjugated mouse anti human CD163 . All experiments performed on human peripheral blood mononuclear cells in the presence of Human SeroBlock (BUF070A).)
Testing Data
(Figure A. RPE conjugated mouse anti CD14 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti CD14 and FITC conjugated mouse anti human CD163 . All experiments performed on human peripheral blood mononuclear cells in the presence of Human SeroBlock (BUF070A).)
Testing Data
(Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD163 . All experiments performed on red cell lysed human blood gated on monocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD163 . All experiments performed on red cell lysed human blood gated on monocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power.)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power.)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Medium power.)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Medium power.)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . High power.)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . High power.)
Immunofluorescence (IF)
(Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.)
Immunofluorescence (IF)
(Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.)
Immunofluorescence (IF)
(Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power.)
Immunofluorescence (IF)
(Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power.)
Immunofluorescence (IF)
(Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power.)
Immunofluorescence (IF)
(Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power.)
Testing Data
(Published customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of infiltrating macrophages in the pulmonary tissues of cattle by immunohistochemistry on formalin fixed tissue sections.Image caption:Immunohistochemical Labeling of Leukocytes in the Lungs.Shown are the results of IHC labeling for CD3-like immunoreactivity (CD3-li), CD163-li, IBA-1-li, and IL-17-li in the lung of uninfected control and representative infected Holstein calves.Note: CD3-li, CD163-li, and IL-17-li is significantly increased in the lungs of the infected calf.Furthermore, CD3+, CD163+, IBA-1+ and IL-17+ mononuclear cells are components of vasculitis lesions. Vascular endothelial cells consistently exhibit pronounced IL-17-li in the infected calf, but endothelial IL-17-li is rare in the control calf. Scale bar: 200 mum.)
Testing Data
(Published customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of infiltrating macrophages in the pulmonary tissues of cattle by immunohistochemistry on formalin fixed tissue sections.Image caption:Immunohistochemical Labeling of Leukocytes in the Lungs.Shown are the results of IHC labeling for CD3-like immunoreactivity (CD3-li), CD163-li, IBA-1-li, and IL-17-li in the lung of uninfected control and representative infected Holstein calves.Note: CD3-li, CD163-li, and IL-17-li is significantly increased in the lungs of the infected calf.Furthermore, CD3+, CD163+, IBA-1+ and IL-17+ mononuclear cells are components of vasculitis lesions. Vascular endothelial cells consistently exhibit pronounced IL-17-li in the infected calf, but endothelial IL-17-li is rare in the control calf. Scale bar: 200 mum.)
Testing Data
(Publised customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.Image caption:Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D.Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i).The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n).Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 mum, (b), (c) to (v) 5 mum.From: Schultz, N. et al. (2016).Amylin alters human brain pericyte viability and NG2 expression.J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Publised customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.Image caption:Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D.Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i).The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n).Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 mum, (b), (c) to (v) 5 mum.From: Schultz, N. et al. (2016).Amylin alters human brain pericyte viability and NG2 expression.J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: integral to plasma membrane; plasma membrane; extracellular region
Molecular Function: protein binding; scavenger receptor activity
Biological Process: receptor-mediated endocytosis; acute-phase response
Research Articles on CD163
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Product Notes
The CD163 cd163 (Catalog #AAA225636) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD163: RPE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD163 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD163 cd163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD163, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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