Mouse CD163 Monoclonal Antibody | anti-CD163 antibody

MOUSE ANTI RAT CD163:Low Endotoxin

Cat. Num. AAA224525

• Gene Names: CD163; M130; MM130
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: CD163; Monoclonal Antibody; MOUSE ANTI RAT CD163:Low Endotoxin; anti-CD163 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: ED2
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1116

• Applicable Applications for anti-CD163 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul ; Immunohistology - Paraffin: Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Histology Positive Control Tissue: Liver; Buffer Solution
• Target Species: Rat
• Preparation and Storage: Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC (MBS216353))

Testing Data #2

(Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE (MBS216355))

Testing Data #3

(Staining of acetone fixed, cryostat sectioned rat spleen with Mouse anti Rat CD163 (MBS216354))

Related Product Information for anti-CD163 antibody

Mouse anti rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.

NCBI and Uniprot Product Information

• NCBI GI #: 5107945
• NCBI GeneID: 9332
• UniProt Accession #: Q86VB7; Q07898; Q07899; Q07900; Q07901; Q2VLH7; C9JIG2
• Molecular Weight: 124,958 Da
• NCBI Official Full Name: CD163
• NCBI Official Synonym Full Names: CD163 molecule
• NCBI Official Symbol: CD163
• NCBI Official Synonym Symbols: M130; MM130
• NCBI Protein Information: scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
• UniProt Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• Protein Family: Scavenger receptor cysteine-rich type 1 protein
• UniProt Gene Name: CD163
• UniProt Synonym Gene Names: M130; sCD163
• UniProt Entry Name: C163A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; acute-phase response