Mouse anti-Porcine CD163 Monoclonal Antibody | anti-CD163 antibody

CD163 (CD163 Antigen, Hemoglobin Scavenger Receptor, M130, M130 Antigen Precursor, Macrophage-associated Antigen, MM130, Scavenger Receptor Cysteine-rich Type 1 Protein M130) (Biotin)

Cat. Num. AAA6248536

• Reactivity: Porcine
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by protein A affinity chromatography from tissue culture supernatant.
• Synonyms: CD163; Monoclonal Antibody; CD163 (CD163 Antigen; Hemoglobin Scavenger Receptor; M130; M130 Antigen Precursor; Macrophage-associated Antigen; MM130; Scavenger Receptor Cysteine-rich Type 1 Protein M130) (Biotin); CD163 Molecule; anti-CD163 antibody

• Host: Mouse
• Reactivity: Porcine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10B837
• Specificity: Recognizes porcine CD163 at ~120kD in alveolar macrophage cell lysates under non-reducing conditions.
• Purity/Purification: Purified by protein A affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

• Applicable Applications for anti-CD163 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Porcine alveolar macrophages
• Conjugate: Biotin
• Preparation and Storage: Store at -20 degree C.

Testing Data

(Staining of porcine peripheral blood mononuclear cells with C2548-90CA.)

Product Categories/Family for anti-CD163 antibody

Antibodies; Receptors; Scavenger

References

1. Sanchez, M. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation. J. Immunol. 162: 5230-5237. 2. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocyte/ macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens 49: 403-413. 3. Yang, P. et al. (2002) Immune cells in the porcine retina: Distribution, characterization and morphological features. Invest. Opthalmol. Vis. Sci. 43: 1488-1492. 4. Gomez del Moral, M. et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis J. Virol. 73: 2173-2180. 5. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers. Vet. Immunol. Immunopathol. 80: 93-109. 6. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection. Arch. Virol. 148: 2307-2323. 7. Delrue, I. et al. (2010) Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163. BMC Biotechnol. 10: 48. 8. Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver. Stem Cells. 26: 1347-55. 9. Moreno, S.et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2. Vet Res. 41: 76. 10. Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae. Vet Res. 41: 64. 11. Urbieta Caceres, V.H. et al. (2011) Early experimental hypertension preserves the myocardial microvasculature but aggravates cardiac injury distal to chronic coronary artery obstruction. Am J Physiol Heart Circ Physiol. 300: H693-701. 12. Das, P.B. et al. (2010) The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163. J Virol. 84: 1731-40. 13. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.