Mouse CD161 Monoclonal Antibody | anti-CD161 antibody

Anti-Hu CD161 Purified

Cat. Num. AAA1800756

• Gene Names: KLRB1; NKR; CD161; CLEC5B; NKR-P1; NKRP1A; NKR-P1A; hNKR-P1A
• Reactivity: Non-human primates, Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: CD161; Monoclonal Antibody; Anti-Hu CD161 Purified; killer cell lectin like receptor B1; NKRP1A; KLRB1; CLEC5B; anti-CD161 antibody

• Host: Mouse
• Reactivity: Non-human primates, Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: HP-3G10
• Specificity: The mouse monoclonal antibody HP-3G10 recognizes an extracellular epitope of CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 225

• Applicable Applications for anti-CD161 antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC/FACS: QC tested
• Immunogen: human NK cells
• Preparation and Storage: Store at 2-8 degree C.

Flow Cytometry (FC/FACS)

(Separation of human CD161 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD161 (HP-3G10) purified antibody (concentration in sample 4 ug/ml) GAM APC.)

Related Product Information for anti-CD161 antibody

CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.

NCBI and Uniprot Product Information

• NCBI GI #: 4504879
• NCBI GeneID: 3820
• NCBI Accession #: NP_002249.1
• NCBI GenBank Nucleotide #: NP_002249.1
• UniProt Accession #: Q12918
• NCBI Official Full Name: killer cell lectin-like receptor subfamily B member 1
• NCBI Official Synonym Full Names: killer cell lectin like receptor B1
• NCBI Official Symbol: KLRB1
• NCBI Official Synonym Symbols: NKR; CD161; CLEC5B; NKR-P1; NKRP1A; NKR-P1A; hNKR-P1A
• NCBI Protein Information: killer cell lectin-like receptor subfamily B member 1
• UniProt Protein Name: Killer cell lectin-like receptor subfamily B member 1
• UniProt Gene Name: KLRB1
• UniProt Synonym Gene Names: CLEC5B; NKRP1A; NKR-P1A
• UniProt Entry Name: KLRB1_HUMAN

NCBI Description

Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. [provided by RefSeq, Jul 2008]

Uniprot Description

KLRB1: Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: plasma membrane; integral to membrane

Molecular Function: transmembrane receptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction