Mouse CD16 / Fc-gamma Receptor III Monoclonal Antibody | anti-FCGR3A antibody

CD16 / Fc-gamma Receptor III Mouse Monoclonal Antibody

Cat. Num. AAA438590

• Gene Names: FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• Reactivity: Human.; Others not tested
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD16 / Fc-gamma Receptor III; Monoclonal Antibody; CD16 / Fc-gamma Receptor III Mouse Monoclonal Antibody; FcRIIIa; FCR-10; FcRIII; FCGRIII; FcR-10; Fc fragment of IgG; low affinity IIIa; receptor for (CD16); neutrophil-specific antigen NA; CD16a; low affinity immunoglobulin gamma Fc region receptor III-A; Fc-gamma RIIIa; immunoglobulin G Fc receptor III; Fc-gamma RIII-alpha; Fc-gamma receptor IIIb (CD16); CD16a antigen; Fc-gamma receptor III-2 (CD 16); FCGR3; Fc gamma receptor III-A; FCG3; low affinity III; Fc-gamma RIII; IGFR3FcgammaRIIIA; CD16FCRIIIA; FCRIII; receptor (CD16a); anti-FCGR3A antibody

• Host: Mouse
• Reactivity: Human.; Others not tested
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: HO-80
• Specificity: It recognizes CD16 (Fc gamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fc gamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 290

• Applicable Applications for anti-FCGR3A antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Functional Studies (Order Ab without BSA & Azide); Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: PBL s from a NK-leukemia patient
• Hu-Chromosome Location: 1q23
• Positive Control: K-562 or U-937 cells. Lymph nodes and tonsils.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Knapp W. et al. (eds) Leukocyte Typing IV, Oxford University Press, Oxford, 1989. Lanier LL et al. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med 1985, 162(6):2089-2106

NCBI and Uniprot Product Information

• NCBI GI #: 50726979
• NCBI GeneID: 2214
• NCBI Accession #: NP_000560.5
• NCBI GenBank Nucleotide #: NM_000569.6
• UniProt Accession #: P08637; Q53FJ0; Q53FL6; Q5EBR4; Q65ZM6; Q6PIJ0; A2N6W9
• Molecular Weight: 50-80kDa
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor III-A isoform a
• NCBI Official Synonym Full Names: Fc fragment of IgG receptor IIIa
• NCBI Official Symbol: FCGR3A
• NCBI Official Synonym Symbols: CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-A
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-A
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: FCGR3A
• UniProt Synonym Gene Names: CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
• UniProt Entry Name: FCG3A_HUMAN

NCBI Description

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: external side of plasma membrane; plasma membrane

Biological Process: immune response; regulation of immune response

Disease: Immunodeficiency 20