Mouse CD16 Monoclonal Antibody | anti-CD16 antibody

anti-human CD16-Biotin

Cat. Num. AAA666159

• Gene Names: FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• Reactivity: Human, Monkey
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: CD16; Monoclonal Antibody; anti-human CD16-Biotin; Monoclonal anti-human CD16(FcgammaRIII)/Biotin; anti-CD16 antibody

• Host: Mouse
• Reactivity: Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3G8
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 233

• Applicable Applications for anti-CD16 antibody: Flow Cytometry (FC/FACS), (Not WB)
• Performance: Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and incubated 45 minutes on ice with 80 mul of anti-CD16/Biotin at 5 mug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. A net 19% sub population of the cells stained positive with a mean shift of 2.4 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD16 antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human polymorphonuclear leukocytes
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended

Testing Data

Related Product Information for anti-CD16 antibody

Information: Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphtidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages. Antibody 3G8 recognizes both allelic forms of CD16. Antibody 3G8 blocks binding of complexed IgG to CD16.

References

H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA 79: 3275-3279. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597. G. Trinchieri & N. Valiante, (1993) Natural Immunity 12: 218-234. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 805-814. J.C. Edberg & R.P. Kimberly, (1997) J Immunol 159: 3849-3857.

NCBI and Uniprot Product Information

• NCBI GI #: 8176551
• NCBI GeneID: 2214
• Molecular Weight: 29,089 Da
• NCBI Official Full Name: CD16, partial
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• NCBI Official Symbol: FCGR3A
• NCBI Official Synonym Symbols: CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-A; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-gamma receptor III-2 (CD 16); immunoglobulin G Fc receptor III; Fc fragment of IgG, low affinity III, receptor for (CD16)
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-A
• Protein Family: CD160 antigen
• UniProt Gene Name: FCGR3A
• UniProt Synonym Gene Names: CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
• UniProt Entry Name: FCG3A_HUMAN

NCBI Description

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: IgG binding

Biological Process: regulation of immune response; innate immune response; immune response

Disease: Immunodeficiency 20