Testing Data
Rat anti-Mouse CD16,32 Monoclonal Antibody
CD16,32 (FITC)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
PurifiedPurified
Synonyms
CD16; 32; Monoclonal Antibody; 32 (FITC); Anti -CD16; anti-CD16; 32 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
93
Specificity
Recognizes murine CD16/32 (CD16/Fc-gammaII and CD32/Fc-gIII receptors). Recognizes a conformational epitope formed by Fc-gII and Fc-gIII receptors.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
Applicable Applications for anti-CD16,32 antibody
Flow Cytometry (FC/FACS)
Application Notes
Flow cytometry 4, 7, 8, , Blocking of Fc-g receptors
Dilution: Flow Cytometry: 3ug/10e6 cells
Dilution: Flow Cytometry: 3ug/10e6 cells
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
Testing Data
Related Product Information for anti-CD16,32 antibody
The lymphocyte Fc-gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta.1, 2 The Fc-g type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios.1 The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.4 Besides identifying Fc-gammaR+ cells, monoclonal antibodies to the Fc-gII/III receptor have been used to block Fc receptor binding of IgG, Fc-mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc-gR for parasitic invasion.4-6
Product Categories/Family for anti-CD16,32 antibody
Similar Products
Product Notes
The CD16,32 (Catalog #AAA607949) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD16,32 (FITC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD16,32 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow cytometry 4, 7, 8,, Blocking of Fc-g receptors Dilution: Flow Cytometry: 3ug/10e6 cells. Researchers should empirically determine the suitability of the CD16,32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16,32, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
