Mouse anti-Human CD15/ Lewis X antigen/ alpha-(1,3)-fucosyltransferase 4 Monoclonal Antibody | anti-CD15 antibody
CD15/ Lewis X antigen/ alpha-(1,3)-fucosyltransferase 4
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
Synonyms
CD15/ Lewis X antigen/ alpha-(1; 3)-fucosyltransferase 4; Monoclonal Antibody; anti-CD15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM
Clone Number
BRA4F1
Specificity
The antibody BRA4F1 is directed against human CD15. The antibody does not cross react with the sialylated form of CD15 (CD15s).
Form/Format
Each vial contains 100 ul 1mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. The product is delivered in lyophilised format.
Sequence Length
530
Applicable Applications for anti-CD15 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC-frozen), Immunohistochemistry (IHC-paraffin), Immunocytochemistry (ICC)
Application Notes
The BRA4F1 antibody is suitable for the detection of CD15 by flow cytometry, immunocytochemisty and immunohistochemistry of frozen and paraffin embedded tissues.
Optimal antibody dilutions for the different applications should be determined by titration.
Optimal antibody dilutions for the different applications should be determined by titration.
Source Note
BRA4F1 is a mouse IgM monoclonal antibody derived from the fusion of SP2.0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with K562 cells.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-CD15 antibody
CD15 is a carbohydrate that is referred to as the Lewis X antigen, also known as alpha-(1,3)-fucosyltransferase 4, which is expressed on a variety of glycolipids and glycoproteins found on the cell surface of neutrophils, monocytes, eosinophils and non-hematopoietic tissues including embryonic tissues. CD15 is reported to function as an adhesion molecule and is involved in chemotaxis and phagocytosis. Expression of CD15 is also associated with a wide variety of tumour cells including some adenocarcinomas and certain types of myeloid and lymphoid leukaemias. Reed-Sternberg cells, found in cases of Hodgkins Lymphoma, also express CD15 and antibodies recognising the CD15 antigen are often used to help differentiate between Hodgkins and non-Hodgkins lymphoma.
Product Categories/Family for anti-CD15 antibody
References
Chorváth B, Sedlák J, Suliková M, Plesková I, Augustínová M, Duraj J. Monoclonal antibodies (series Bra-) of broad (non-lineage) or restricted (myelomonocytic) reactivities elicited with granulocytes or K 562 cells. Neoplasma. 1989;36(6):633-42.
2. CD15 workshop In Leucocyte Typing IV. White Cell Differentiation Antigens - International workshop and conference. Knapp W, Doeken B, Gilks EP, Schmidt RE, Stein H, von dem Borne A.E.G.Kr. Eds. Vienna, Oxford University Press.1989.
2. CD15 workshop In Leucocyte Typing IV. White Cell Differentiation Antigens - International workshop and conference. Knapp W, Doeken B, Gilks EP, Schmidt RE, Stein H, von dem Borne A.E.G.Kr. Eds. Vienna, Oxford University Press.1989.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,084 Da
NCBI Official Full Name
alpha-(1,3)-fucosyltransferase 4
NCBI Official Synonym Full Names
fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
NCBI Official Symbol
FUT4
NCBI Official Synonym Symbols
LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 4; alpha-(1,3)-fucosyltransferase 4; Lewis X; fucT-IV; fucosyltransferase IV; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; galactoside 3-L-fucosyltransferase; stage-specific embryonic antigen 1
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 4
UniProt Gene Name
FUT4
UniProt Synonym Gene Names
ELFT; FCT3A; Fuc-TIV; FucT-IV
UniProt Entry Name
FUT4_HUMAN
NCBI Description
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Uniprot Description
FUT4: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. Belongs to the glycosyltransferase 10 family.
Protein type: Membrane protein, integral; Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.-
Chromosomal Location of Human Ortholog: 11q21
Cellular Component: Golgi apparatus; cell surface; membrane; integral to membrane
Molecular Function: alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity
Biological Process: carbohydrate metabolic process; protein amino acid glycosylation; L-fucose catabolic process
Research Articles on CD15
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Product Notes
The CD15 fut4 (Catalog #AAA570325) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD15/ Lewis X antigen/ alpha-(1,3)-fucosyltransferase 4 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD15/ Lewis X antigen/ alpha-(1,3)-fucosyltransferase 4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC-frozen), Immunohistochemistry (IHC-paraffin), Immunocytochemistry (ICC). The BRA4F1 antibody is suitable for the detection of CD15 by flow cytometry, immunocytochemisty and immunohistochemistry of frozen and paraffin embedded tissues. Optimal antibody dilutions for the different applications should be determined by titration. Researchers should empirically determine the suitability of the CD15 fut4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD15/ Lewis X antigen/ alpha-(1,3)-fucosyltransferase 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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