Immunohistochemistry (IHC)
(Figure 1: Immunohistochemical analysis of paraffin-embedded human cerebellum tissues (left) and human liver cancer tissues (right) using CD15 mouse mAb with DAB staining.)
Mouse anti-Human CD15 Monoclonal Antibody | anti-CD15 antibody
CD15 Antibody
Gene Names
CD151; GP27; MER2; RAPH; SFA1; PETA-3; TSPAN24
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
CD15; Monoclonal Antibody; CD15 Antibody; CD151; CD151 antigen; CD151 molecule (Raph blood group); CD151 molecule; CD151_HUMAN; GP27; hemidesmosomal tetraspanin CD151; Membrane glycoprotein SFA-1; MER2; PETA-3; PETA3; Platelet endothelial tetraspan antigen 3; platelet surface glycoprotein gp27; Platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; Red blood cell antigen MER2; SFA 1; SFA1; Tetraspanin-24; Tspan-24; TSPAN24; anti-CD15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
CD15 antibody detects endogenous levels of total CD15
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
253
Applicable Applications for anti-CD15 antibody
Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
ELISA: 1:10000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
Immunogen
Purified recombinant fragment of human CD15 expressed in E. Coli
Subcellular Location
Membrane.
Tissue Specificity
Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(Figure 1: Immunohistochemical analysis of paraffin-embedded human cerebellum tissues (left) and human liver cancer tissues (right) using CD15 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Figure 1: Immunohistochemical analysis of paraffin-embedded human cerebellum tissues (left) and human liver cancer tissues (right) using CD15 mouse mAb with DAB staining.)
Related Product Information for anti-CD15 antibody
Description: The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures.
Function: Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
Subunit Structure: Interacts with integrins ITGA3:ITGB1, ITGA5:ITGB1, ITGA3:ITGB1 and ITGA6:ITGB4 and with CD9 and CD181 (PubMed:11907260). Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (PubMed:27993971).
Similarity: Belongs to the tetraspanin (TM4SF) family.
Function: Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
Subunit Structure: Interacts with integrins ITGA3:ITGB1, ITGA5:ITGB1, ITGA3:ITGB1 and ITGA6:ITGB4 and with CD9 and CD181 (PubMed:11907260). Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (PubMed:27993971).
Similarity: Belongs to the tetraspanin (TM4SF) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 59 kDa
Predicted: 29 kDa
Predicted: 29 kDa
NCBI Official Full Name
CD151 antigen
NCBI Official Synonym Full Names
CD151 molecule (Raph blood group)
NCBI Official Symbol
CD151
NCBI Official Synonym Symbols
GP27; MER2; RAPH; SFA1; PETA-3; TSPAN24
NCBI Protein Information
CD151 antigen
UniProt Protein Name
CD151 antigen
Protein Family
UniProt Gene Name
CD151
UniProt Synonym Gene Names
TSPAN24; PETA-3; Tspan-24
NCBI Description
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
Research Articles on CD15
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Product Notes
The CD15 cd151 (Catalog #AAA9602519) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD15 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD15 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). ELISA: 1:10000 IHC: 1:200-1:1000 ICC: 1:200-1:1000. Researchers should empirically determine the suitability of the CD15 cd151 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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