Mouse anti-Human CD148 Monoclonal Antibody

CD148 (Receptor-type Tyrosine-protein Phosphatase eta, Protein-tyrosine Phosphatase eta, R-PTP-eta, Density-enhanced Phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine Phosphatase Receptor Type J, R-PTP-J, PTPRJ, DEP1)

Cat. Num. AAA649772

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CD148; Monoclonal Antibody; CD148 (Receptor-type Tyrosine-protein Phosphatase eta; Protein-tyrosine Phosphatase eta; R-PTP-eta; Density-enhanced Phosphatase 1; DEP-1; HPTP eta; Protein-tyrosine Phosphatase Receptor Type J; R-PTP-J; PTPRJ; DEP1); Anti -CD148 (Receptor-type Tyrosine-protein Phosphatase eta; anti-CD148 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: A3
• Specificity: Recognizes human CD148.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CD148 antibody: Flow Cytometry (FC/FACS). Other applications have not tested.
• Application Notes: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunoprecipitation.; Dilution: Flow Cytometry: < or = 1ug/10e6 cells in 100ul volume or whole blood. ; Optimal dilutions to be detemined by the researcher.
• Immunogen: PHA-stimulated human PBMC
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Human peripheral blood granulocytes stained with MBS649772, followed by anti-mouse IgG FITC.)

Related Product Information for anti-CD148 antibody

CD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leukocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leukocytes. CD148 as a leukocyte activation marker and may be involved in the regulation of T cell activation. Leukocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors.

Product Categories/Family for anti-CD148 antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• UniProt Accession #: Q12913