Mouse anti-Human CD147 Monoclonal Antibody | anti-CD147 antibody

Mouse anti-Human CD147, FITC Conjugated mAb

Cat. Num. AAA8503807

• Gene Names: BSG; OK; 5F7; TCSF; CD147; EMPRIN; EMMPRIN
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: CD147; Monoclonal Antibody; Mouse anti-Human CD147; FITC Conjugated mAb; anti-CD147 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4AHIM6
• Form/Format: Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
• Sequence Length: 269

• Applicable Applications for anti-CD147 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube; ; 2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light; ; 3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×); ; 4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).; [PBS wash buffer: PBS +1% FBS +0.1% NaN3]; [Cell fixation: 2% formaldehyde solution]
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Related Product Information for anti-CD147 antibody

4AHIM6 monoclonal antibody reacts with basigin or neurothelin, a 50-60 kDa transmembrane glycoprotein, broadly expressed on cells of hematopoietic and non-hematopoietic origin. Neutrothelin is a blood-brain barrier-specific molecule. CD147 play a role in embryonal blood barrier development and a role in integrin-mediated adhesion in brain endothelia.

References

1.Schlossman, SL et al., eds. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York. eds.

NCBI and Uniprot Product Information

• NCBI GI #: 31076333
• NCBI GeneID: 682
• Molecular Weight: 22,764 Da
• NCBI Official Full Name: CD147
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: OK; 5F7; TCSF; CD147; EMPRIN; EMMPRIN
• NCBI Protein Information: basigin
• UniProt Protein Name: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: EMMPRIN; TCSF

NCBI Description

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8 and SLC16A11 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.