Mouse anti-Human CD14 Monoclonal Antibody | anti-CD14 antibody

CD14, Human, mAb 18D11

Cat. Num. AAA584146

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD14; Monoclonal Antibody; Human; mAb 18D11; anti-CD14 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 373

• Applicable Applications for anti-CD14 antibody: Flow Cytometry (FC/FACS), FS
• Application Notes: Application Use: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of human sCD14 to be inactivated.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-CD14 antibody

Product Description: The monoclonal antibody 18D11 recognizes human monocyte marker CD14, a 53 kDa glycoprotein. CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present at low density on a subpopulation granulocytes and activated or transformed B-cells. The monoclonal antibody 18D11 reacts with both soluble and membrane CD14. The monoclonal antibody 18D11 is useful for inhibition of biological activity of sCD14, it inhibits LPS induced TNF production. The monoclonal antibody is cross reactive with pig CD14. Together with a C5a neutralizing monoclonal antibody, 18D11 blocks sepsis in a pig model.

References

1. Tunheim, G et al; Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes. J Leukoc Biol 2005, 77: 303 2. Wang, J et al; Peptidoglycan primes for LPS-induced release of proinflammatory cytokines in whole human blood. Shock 2001, 16: 178
3. Wang, J et al; Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 2001 69: 2402
4. Wang, J et al; Peptidoglycan and lipoteichoic acid from Staphylococcus aureus induce tumor necrosis factor alpha, interleukin 6 (IL-6), and IL-10 production in both T cells and monocytes in a human whole blood model. Infect Immun 2000 68: 3965

NCBI and Uniprot Product Information

• NCBI GI #: 2274810
• NCBI GeneID: 281048
• Molecular Weight: 39,667 Da
• NCBI Official Full Name: CD14
• NCBI Official Synonym Full Names: CD14 molecule<
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: CD14
• UniProt Entry Name: CD14_BOVIN

Uniprot Description

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().