Standard Curve (Sample)
Mouse Immunoglobulin G12a (IgG2a) ELISA Kit | IgG2a elisa kit
Mouse Immunoglobulin G12a (IgG2a) quantitative detection ELISA Kit
Reactivity
Mouse
Synonyms
Immunoglobulin G12a (IgG2a); N/A; Mouse Immunoglobulin G12a (IgG2a) quantitative detection ELISA Kit; IgG2a elisa kit
Reactivity
Mouse
Specificity
This kit identifies natural and recombinant Mouse IgG2A, with No crossing with structural analogs.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, and cell culture supernatant
Sensitivity
< 0.1 ng/ml
Intra-assay Precision
In the same lot CV%<10?3 groups of known high, medium, and low concentration samples were evaluated 20 times on the same plate.
Inter-assay Precision
Different lot CV%<15: 3 groups of known high, medium, and low concentration samples were evaluated 20 times on different plates.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IgG2a elisa kit
Principle of the Assay: This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Mouse IgG2A antibody (solid phase antigen), standards or samples are added to the microtiter plate wells, then add anti-Mouse IgG2A HRP labeled antibody. Wells are thoroughly washed to remove all unbound components. And a sandwich complex of solid antigen-antibody enzyme-labeled secondary antibodies is formed on the solid surface of the microplate. Next, Substrate solution A and substrate solution B are added to each well, and it will produce blue materials under HRP catalysis. The enzyme-substrate reaction is terminated by the addition of sulphuric acid and the color change to yellow. Measured spectrophotometrically at a wavelength of 450 nm, the absorbance (OD value) is positively correlated with the concentration of Mouse IgG2A in the sample. The concentration of IgG2A in the samples could be determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: This kit is used for detecting the Mouse IGg2a concentration of serum, plasma, and cell culture supernatant.
Intended Uses: This kit is used for detecting the Mouse IGg2a concentration of serum, plasma, and cell culture supernatant.
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Product Notes
The Mouse IgG2a (Catalog #AAA210685) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA210685 ELISA Kit recognizes Mouse IgG2a. It is sometimes possible for the material contained within the vial of "Immunoglobulin G12a (IgG2a), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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