Testing Data
(MBS606833 inhibits the 3H-thymidine incorporation by M1 cells in a dose dependant manner (Satio, T. et al., 1989, J. Immunol. 147:168 - 173). The ED for this effect is typically 4-10ng/ml.)
Rat CD130 Monoclonal Antibody
CD130 (Interleukin 6 Receptor beta, gp130, neutralizing, IL-6Rb, Interleukin-6 signal transducer)
Applications
ELISA, Neutralization Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
CD130; Monoclonal Antibody; CD130 (Interleukin 6 Receptor beta; gp130; neutralizing; IL-6Rb; Interleukin-6 signal transducer); Anti -CD130 (Interleukin 6 Receptor beta; anti-CD130 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9H92
Specificity
Recognizes mouse gp130. Does not crossreact with recombinant human gp130, recombinant mouse IL-6 or recombinant mouse IL-6R.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Applicable Applications for anti-CD130 antibody
ELISA (EL/EIA), Neutralization
Application Notes
Dilution: ELISA (Capture): 2-8ug/ml
ELISA (Detection): 0.1-0.4ug/ml
Neutralization: Measured by its' ability to neutralize IL-6 induced inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The ND50 is typically 0.02-0.1ug/ml in the presence of 20ng/ml recombinant human IL-6.
ELISA (Detection): 0.1-0.4ug/ml
Neutralization: Measured by its' ability to neutralize IL-6 induced inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The ND50 is typically 0.02-0.1ug/ml in the presence of 20ng/ml recombinant human IL-6.
Immunogen
Recombinant protein corresponding to aa23-617 of mouse gp130 expressed in NSO cells (Accession #Q00560).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(MBS606833 inhibits the 3H-thymidine incorporation by M1 cells in a dose dependant manner (Satio, T. et al., 1989, J. Immunol. 147:168 - 173). The ED for this effect is typically 4-10ng/ml.)
Testing Data
(MBS606833 inhibits the 3H-thymidine incorporation by M1 cells in a dose dependant manner (Satio, T. et al., 1989, J. Immunol. 147:168 - 173). The ED for this effect is typically 4-10ng/ml.)
Testing Data
(Ability of MBS606833 to block gp130-mediated IL-6 response on mouse M1 cells, added cells to wells in 96 well plate and ncubated at 37° C for 1 hour, rhIL-6 was added. The assay mixture, incubated at 37°C for 2 days in humidified CO2 incubator. 3 H-thymidine was added during the final 4hrs of incubation. The ND of the antibody is ~0.02-0.1ug/ml.)
Testing Data
(Ability of MBS606833 to block gp130-mediated IL-6 response on mouse M1 cells, added cells to wells in 96 well plate and ncubated at 37° C for 1 hour, rhIL-6 was added. The assay mixture, incubated at 37°C for 2 days in humidified CO2 incubator. 3 H-thymidine was added during the final 4hrs of incubation. The ND of the antibody is ~0.02-0.1ug/ml.)
Related Product Information for anti-CD130 antibody
The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component speci c to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low af nity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. CD126 is single chain type I transmembrane protein with Mr=130-140kD and is a common receptor subunit for many cytokines, forming complexes with CD126, IL-11R, LIF-R, and other proteins. These gp130-containing complexes mediate signaling of oncostatin M, IL-6, IL-11, LIF, CNF, and cardiolipotrophin 1. gp130 is expressed at low levels by most cell types. This antibody was recognized as reacting with gp130 at the Sixth Leukocyte Typing Workshop.
Product Categories/Family for anti-CD130 antibody
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Product Notes
The CD130 (Catalog #AAA606833) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD130 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Neutralization. Dilution: ELISA (Capture): 2-8ug/ml ELISA (Detection): 0.1-0.4ug/ml Neutralization: Measured by its' ability to neutralize IL-6 induced inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The ND50 is typically 0.02-0.1ug/ml in the presence of 20ng/ml recombinant human IL-6. Researchers should empirically determine the suitability of the CD130 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD130, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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