Mouse anti-Human CD13 Monoclonal Antibody | anti-CD13 antibody

Anti-Human CD13 Monoclonal Antibody (Alexa Fluor 647 Conjuaged)

Cat. Num. AAA2556883

• Gene Names: ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD13; Monoclonal Antibody; Anti-Human CD13 Monoclonal Antibody (Alexa Fluor 647 Conjuaged); Aminopeptidase N; ANPEP; AP-N; hAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; gp150; anti-CD13 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: B-F10
• Form/Format: Liquid; PBS with 0.05% Proclin300, 1% BSA
• Sequence Length: 967

• Applicable Applications for anti-CD13 antibody: Flow Cytometry (FC/FACS)
• Conjugation: Alexa Fluor 647
• Preparation and Storage: Store at 2-8 degree C and protected from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(Flow cytometry antibody)

Flow Cytometry (FC/FACS)

(Human peripheral blood cells are stained with Anti-Human CD13 Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(Left). Unstained leukocytes are used as control(Right).)

Related Product Information for anti-CD13 antibody

CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. This zinc metallopeptidase is expressed as a homodimer on granulocytes, myeloid progenitors, endothelial cells, epithelial cells and subset of granular lymphoid cells. It is not expressed on platelets or erythrocytes. CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. CD13 serves as the cellular receptor for Coronavirus.

References

1. Shipp M, et al. 1993. Blood 82:1052. 2. Larsen S, et al. 1996. J. Exp. Med. 184:183.

NCBI and Uniprot Product Information

• NCBI GI #: 157266300
• NCBI GeneID: 290
• NCBI Accession #: NP_001141.2
• NCBI GenBank Nucleotide #: NM_001150.3
• UniProt Accession #: P15144
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl aminopeptidase, membrane
• NCBI Official Symbol: ANPEP
• NCBI Official Synonym Symbols: APN; CD13; LAP1; P150; PEPN; GP150
• NCBI Protein Information: aminopeptidase N
• UniProt Protein Name: Aminopeptidase N
• UniProt Gene Name: ANPEP
• UniProt Synonym Gene Names: APN; CD13; PEPN; AP-N; hAPN; AP-M
• UniProt Entry Name: AMPN_HUMAN

NCBI Description

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]

Uniprot Description

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: Receptor, misc.; EC 3.4.11.2; Other Amino Acids Metabolism - glutathione; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 15q25-q26

Cellular Component: extracellular space; lysosomal membrane; integral to plasma membrane; ER-Golgi intermediate compartment; cytosol; external side of plasma membrane

Molecular Function: viral receptor activity; zinc ion binding; metallopeptidase activity; receptor activity; aminopeptidase activity

Biological Process: entry of virus into host cell; cellular protein metabolic process; angiogenesis; angiotensin maturation; cell differentiation; proteolysis