Rat anti-Mouse CD122/IL-2R beta Monoclonal Antibody
Rat Anti-Mouse CD122-APC
Reactivity
Mouse
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
CD122/IL-2R beta; Monoclonal Antibody; Rat Anti-Mouse CD122-APC; Rat Anti-Mouse CD122 - Allophycocyanin; Rat Anti-Mouse CD122/IL-2R beta; anti-CD122/IL-2R beta antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
5H4
Specificity
beta chain (Mr 90-100 kDa) of the IL-2 receptor.
The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the alpha chain which binds IL-2 with low affinity; (ii) the beta chain that binds IL-2 with high affinity; and (ii) the common gamma chain (gamma C) that does not bind IL-2.2 The high affinity receptor complex is an alpha / beta / gamma heterotrimer with a Kd of 1.3 x 10-11 M.2 In mouse spleen, CD122 is expresed on ? 30% of CD8+ cells and all NK cells, but <1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells.4 Cytoplasmic regions of the IL-2R beta chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes.1, 4 The monoclonal antibody 5H4 does not block IL-2 binding.1
The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the alpha chain which binds IL-2 with low affinity; (ii) the beta chain that binds IL-2 with high affinity; and (ii) the common gamma chain (gamma C) that does not bind IL-2.2 The high affinity receptor complex is an alpha / beta / gamma heterotrimer with a Kd of 1.3 x 10-11 M.2 In mouse spleen, CD122 is expresed on ? 30% of CD8+ cells and all NK cells, but <1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells.4 Cytoplasmic regions of the IL-2R beta chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes.1, 4 The monoclonal antibody 5H4 does not block IL-2 binding.1
Form/Format
Allophycocyanin (APC) Conjugate
Applicable Applications for anti-CD122/IL-2R beta antibody
Flow Cytometry; Immunoprecipitation
Application Notes
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Immunogen
Rat myeloma YB2/0 transfected with truncated mouse IL-2RB cDNA
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Malek, T.R., et al. 1995. J. Interferon Cytokine Res. 15:447.
2. Minami, Y., et al. 1993. Annu. Rev. Immunol. 11:245.
3. Taniguchi, T., and Y. Minami. 1993. Cell 73:5.
4. Tanaka, T., et al. 1991. J. Immunol. 147:2222.
2. Minami, Y., et al. 1993. Annu. Rev. Immunol. 11:245.
3. Taniguchi, T., and Y. Minami. 1993. Cell 73:5.
4. Tanaka, T., et al. 1991. J. Immunol. 147:2222.
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Product Notes
The CD122/IL-2R beta (Catalog #AAA673673) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD122-APC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD122/IL-2R beta can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the CD122/IL-2R beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD122/IL-2R beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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