WB (Western Blot)
(Detection of human Nucleobindin 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nucleobindin 1 antibody AAA212967 (lot AAA212967-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Nucleobindin 1/NUCB1 Polyclonal Antibody | anti-NUCB1 antibody
Rabbit anti-Nucleobindin 1/NUCB1 Antibody, Affinity Purified
Gene Names
NUCB1; NUC; CALNUC
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Nucleobindin 1/NUCB1, Antibody; Rabbit anti-Nucleobindin 1/NUCB1 Antibody, Affinity Purified; CALNUC; NUC; nucleobindin-1; Nucleobindin-1; nucleobindin 1; anti-NUCB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NUCB1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 411 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Nucleobindin 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nucleobindin 1 antibody AAA212967 (lot AAA212967-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Nucleobindin 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nucleobindin 1 antibody AAA212967 (lot AAA212967-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Nucleobindin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nucleobindin 1 antibody AAA212967 (lot AAA212967-1) used for IP at 6 ug per reaction. Nucleobindin 1 was also immunoprecipitated by rabbit anti-Nucleobindin 1 antibody A304-332A. For blotting immunoprecipitated Nucleobindin 1, AAA212967 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Nucleobindin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nucleobindin 1 antibody AAA212967 (lot AAA212967-1) used for IP at 6 ug per reaction. Nucleobindin 1 was also immunoprecipitated by rabbit anti-Nucleobindin 1 antibody A304-332A. For blotting immunoprecipitated Nucleobindin 1, AAA212967 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-NUCB1 antibody
Nucleobindin 1 is a member of a small calcium-binding EF-hand protein family. Nucleobindin 1 is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events [taken from NCBI Entrez Gene (Gene ID: 4924)].
Product Categories/Family for anti-NUCB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,879 Da
NCBI Official Full Name
nucleobindin-1
NCBI Official Synonym Full Names
nucleobindin 1
NCBI Official Symbol
NUCB1
NCBI Official Synonym Symbols
NUC; CALNUC
NCBI Protein Information
nucleobindin-1
UniProt Protein Name
Nucleobindin-1
UniProt Gene Name
NUCB1
UniProt Synonym Gene Names
NUC
UniProt Entry Name
NUCB1_HUMAN
Similar Products
Product Notes
The NUCB1 nucb1 (Catalog #AAA212967) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Nucleobindin 1/NUCB1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleobindin 1/NUCB1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NUCB1 nucb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleobindin 1/NUCB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.