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Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MBS210091), red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Mouse CD11b Monoclonal Antibody | anti-CD11b antibody

MOUSE ANTI RAT CD11b:FITC

Applications
Flow Cytometry, Functional Assay
Synonyms
CD11b; Monoclonal Antibody; MOUSE ANTI RAT CD11b:FITC; anti-CD11b antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ED8
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Applicable Applications for anti-CD11b antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Immunogen
Rat spleen cell homogenate with FCA
Fusion Partners
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MBS210091), red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MBS210091), red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Testing Data

(Published customer image: Lithium inhibits lipopolysaccharide (LPS)-induced microglial activation. Primary microglial cells were pretreated with LiCl at 0.3, 1 and 3 mM for 30 minutes and then stimulated with LPS at 10 ng/ml for 24 hours. (a) and (b) For flow cytometric analysis, the cells were incubated with PE-conjugated ED8 antibody at 37 degree C for 1 hour. **P)

Testing Data (Published customer image: Lithium inhibits lipopolysaccharide (LPS)-induced microglial activation. Primary microglial cells were pretreated with LiCl at 0.3, 1 and 3 mM for 30 minutes and then stimulated with LPS at 10 ng/ml for 24 hours. (a) and (b) For flow cytometric analysis, the cells were incubated with PE-conjugated ED8 antibody at 37 degree C for 1 hour. **P)

Testing Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power)

Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power)

Testing Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MBS210091), red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MBS210091), red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Testing Data

(Published customer image: TLR4 regulates microglial activation. Primary microglial cells were transfected with TLR4 siRNA (100 nM) or control (siControl) for 48 hours and then stimulated with lipopolysaccharide (LPS) (10 ng/ml) for 24 hours. (a) Suppression of TLR4 expression by TLR4 siRNA. (b) Quantification of IL-6 and TNF-a production in media, **P)

Testing Data (Published customer image: TLR4 regulates microglial activation. Primary microglial cells were transfected with TLR4 siRNA (100 nM) or control (siControl) for 48 hours and then stimulated with lipopolysaccharide (LPS) (10 ng/ml) for 24 hours. (a) Suppression of TLR4 expression by TLR4 siRNA. (b) Quantification of IL-6 and TNF-a production in media, **P)

Testing Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Low power)

Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Low power)

Testing Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power)

Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MBS210091) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power)
Related Product Information for anti-CD11b antibody
Mouse anti Rat CD11b antibody, clone ED8 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer (160 and 95kD) belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3. Mouse anti Rat CD11b antibody, clones ED7 and ED8 may recognize closely related epitopes on the same molecule. ED7 and ED8 have been shown to induce homotypic aggregation of granulocytes.

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Product Notes

The CD11b (Catalog #AAA211067) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD11b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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